概述

  • 产品名称Anti-DDX18抗体
    参阅全部 DDX18 一抗
  • 描述
    兔多克隆抗体to DDX18
  • 特异性We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Baboon, Orangutan
  • 免疫原

    A region between residue 625 and the C terminus (residue 670) of human DDX18 (NP_006764.3)

  • 阳性对照
    • Whole cell lysate from HeLa and 293T cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab70527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 1 - 4 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Probable RNA-dependent helicase.
    • 序列相似性Belongs to the DEAD box helicase family. DDX18/HAS1 subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ATP dependent RNA helicase DDX18 antibody
      • ATP-dependent RNA helicase DDX18 antibody
      • DDX 18 antibody
      • DDX18 antibody
      • DDX18_HUMAN antibody
      • DEAD (Asp Glu Ala Asp) box polypeptide 18 antibody
      • DEAD box polypeptide 18 antibody
      • DEAD box protein 18 antibody
      • FLJ33908 antibody
      • MrDb antibody
      • Myc regulated DEAD box protein antibody
      • Myc-regulated DEAD box protein antibody
      see all

    Anti-DDX18 antibody 图像

    • All lanes : Anti-DDX18 antibody (ab70527) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg

      Developed using the ECL technique

      Predicted band size : 75 kDa
      Observed band size : 75 kDa


      Exposure time : 3 minutes
    • Detection of DDX18 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab70526 (Lane 1) and ab70527 (Lane 2) at 3 µg/mg lysate for IP. ab70527 was used at 1 µg/ml for subsequent WB detection. Lane 3 represents control IgG IP.
      Chemiluminescence with an exposure time of 30 seconds.

    Anti-DDX18 antibody (ab70527)参考文献

    ab70527 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab70527.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"