概述

  • 产品名称
  • 描述
    兔多克隆抗体to DDX18
  • 特异性
    We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Cow, Dog, Pig, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    A region between residues 1 and 50 of human DDX18 (NP_006764.3)

  • 阳性对照
    • Whole cell lysate from HeLa and 293T cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab70526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明
    IP: Use at 1 - 4 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Probable RNA-dependent helicase.
    • 序列相似性
      Belongs to the DEAD box helicase family. DDX18/HAS1 subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ATP dependent RNA helicase DDX18 antibody
      • ATP-dependent RNA helicase DDX18 antibody
      • DDX 18 antibody
      • DDX18 antibody
      • DDX18_HUMAN antibody
      • DEAD (Asp Glu Ala Asp) box polypeptide 18 antibody
      • DEAD box polypeptide 18 antibody
      • DEAD box protein 18 antibody
      • FLJ33908 antibody
      • MrDb antibody
      • Myc regulated DEAD box protein antibody
      • Myc-regulated DEAD box protein antibody
      see all

    Anti-DDX18 antibody 图像

    • All lanes : Anti-DDX18 antibody (ab70526) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg

      Developed using the ECL technique

      Predicted band size : 75 kDa
      Observed band size : 75 kDa


      Exposure time : 3 minutes
    • Detection of DDX18 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab70526 (Lane 1) and ab70527 (Lane 2) at 3 µg/mg lysate for IP. ab70526 was used at 1 µg/ml for subsequent WB detection. Lane 3 represents control IgG IP.

    Anti-DDX18 antibody (ab70526)参考文献

    ab70526 has not yet been referenced specifically in any publications.

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