Anti-DDX11抗体[3C1] (ab88072)

概述

  • 产品名称Anti-DDX11抗体[3C1]
    参阅全部 DDX11 一抗
  • 描述
    小鼠单克隆抗体[3C1] to DDX11
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length Human DDX11 with a 26 kDa tag (AAH11264)

  • 阳性对照
    • Human liver tissue lysate HeLa cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab88072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 108 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能DNA helicase involved in cellular proliferation. Possesses DNA-dependent ATPase and helicase activities. This helicase translocates on single-stranded DNA in the 5' to 3' direction in the presence of ATP and, to a lesser extent, dATP. Its unwinding activity requires a 5'-single-stranded region for helicase loading, since flush-ended duplex structures do not support unwinding. The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended to 500 bp by RPA or the cohesion establishment factor, the Ctf18-RFC (replication factor C) complex activities. Stimulates the flap endonuclease activity of FEN1. Required for normal sister chromatid cohesion. Required for recruitement of bovine papillomavirus type 1 regulatory protein E2 to mitotic chrmosomes and for viral genome maintenance. Required for maintaining the chromosome segregation and is essential for embryonic development and the prevention of aneuploidy. May function during either S, G2, or M phase of the cell cycle. Binds to both single- and double-stranded DNA.
  • 组织特异性Highly expressed in spleen, B-cells, thymus, testis, ovary, small intestine, and pancreas. Very low expression seen in the brain. Expressed in dividing cells and/or cells undergoing high levels of recombination. No expression is seen in cells signaled to terminally differentiate. Expressed in keratinocyte growth factor-stimulated cells but not in serum, EGF and IL1-beta-treated keratinocytes.
  • 疾病相关Defects in DDX11 are the cause of Warsaw breakage syndrome (WBRS) [MIM:613398]. It is a syndrome characterized by severe microcephaly, pre- and postnatal growth retardation, facial dysmorphism and abnormal skin pigmentation. Additional features include high arched palate, coloboma of the right optic disk, deafness, ventricular septal defect, toes and fingers abnormalities. At cellular level, drug-induced chromosomal breakage, a feature of Fanconi anemia, and sister chromatid cohesion defects, a feature of Roberts syndrome, coexist.
  • 序列相似性Belongs to the DEAD box helicase family. DEAH subfamily. DDX11/CHL1 sub-subfamily.
    Contains 1 helicase ATP-binding domain.
  • 细胞定位Nucleus. Nucleus > nucleolus. During the early stages of mitosis, localizes to condensed chromatin and is released from the chromatin with progression to metaphase. Also localizes to the spindle poles throughout mitosis and at the midbody at later stages of mitosis (metaphase to telophase). Co-localizes with bovine papillomavirus type 1 regulatory protein E2 at early stages of mitosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CHL1 antibody
    • CHL1 related helicase gene 1 antibody
    • CHL1-like helicase homolog antibody
    • CHL1-related protein 1 antibody
    • CHLR1 antibody
    • Ddx11 antibody
    • DDX11_HUMAN antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 11 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (CHL1-like helicase homolog, S. cerevisiae) antibody
    • DEAD/H box antibody
    • DEAD/H box protein 11 antibody
    • hCHLR1 antibody
    • Keratinocyte growth factor regulated gene 2 protein antibody
    • Keratinocyte growth factor-regulated gene 2 protein antibody
    • KRG 2 antibody
    • KRG-2 antibody
    • KRG2 antibody
    • MGC133249 antibody
    • MGC9335 antibody
    • OTTHUMP00000238778 antibody
    • OTTHUMP00000238780 antibody
    • OTTHUMP00000238784 antibody
    • OTTHUMP00000238785 antibody
    • Probable ATP dependent RNA helicase DDX11 antibody
    • Probable ATP-dependent RNA helicase DDX11 antibody
    • WABS antibody
    see all

Anti-DDX11 antibody [3C1] 图像

  • Anti-DDX11 antibody [3C1] (ab88072) at 1 µg/ml + Immunogen (recombinant fragment) at 0.2 µg

    Secondary
    Goat anti mouse IgG at 1/5000 dilution

    Predicted band size : 108 kDa
  • Anti-DDX11 antibody [3C1] (ab88072) at 1 µg/ml + Human liver tissue lysate at 50 µg

    Secondary
    Goat anti-mouse IgG at 1/5000 dilution

    Predicted band size : 108 kDa
    Observed band size : 108 kDa
  • Anti-DDX11 antibody [3C1] (ab88072) at 1 µg/ml + HeLa cell lysate at 50 µg

    Secondary
    Goat anti-mouse IgG at 1/5000 dilution

    Predicted band size : 108 kDa
    Observed band size : 108 kDa

Anti-DDX11 antibody [3C1] (ab88072)参考文献

ab88072 has not yet been referenced specifically in any publications.

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