概述

  • 产品名称Anti-DDX1抗体
    参阅全部 DDX1 一抗
  • 描述
    兔多克隆抗体to DDX1
  • 经测试应用适用于: WB, IHC-P, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 650 and the C-terminus (residue 697) of human DDX1. (AAH12739.1)

  • 阳性对照
    • Whole cell lysate from Hela cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab70252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 3-5 µg/mg of lysate.

靶标

  • 功能Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation. Acts as a positive transcriptional regulator of cyclin CCND2 expression. Binds to the cyclin CCND2 promoter region. Associates with chromatin at the NF-kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Required for HIV-1 Rev function as well as for HIV-1 replication. Binds to the RRE sequence of HIV-1 mRNAs.
  • 组织特异性Highest levels of transcription in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin including the retina, brain, and spinal cord.
  • 序列相似性Belongs to the DEAD box helicase family. DDX1 subfamily.
    Contains 1 B30.2/SPRY domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 结构域The helicase domain is involved in the stimulation of RELA transcriptional activity.
  • 翻译后修饰Phosphorylated. Phosphorylated by ATM kinase; phosphorylation is increased in response to ionizing radiation (IR).
  • 细胞定位Nucleus. Cytoplasm. Cytoplasmic granule. Localized with MBNL1, TIAL1 and YBX1 in stress granules upon stress. Localized with CSTF2 in cleavage bodies. Forms large aggregates called DDX1 bodies. Relocalized into multiple foci (IR-induced foci or IRIF) after IR treatment, a process that depends on the presence of chromosomal DNA and/or RNA-DNA duplexes. Relocalized at sites of DNA double-strand breaks (DSBs) in an ATM-dependent manner after IR treatment. Colocalized with RELA in the nucleus upon TNF-alpha induction. Relocalized to the cytoplasm with a perinuclear staining pattern in avian infectious bronchitis virus (IBV)-infected cells. Required for proper localization of HIV-1 Rev.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP dependent helicase DDX1 antibody
    • ATP-dependent RNA helicase DDX1 antibody
    • DBP RB antibody
    • DBP-RB antibody
    • DDX1 antibody
    • DDX1_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 1 antibody
    • DEAD box 1 antibody
    • DEAD box polypeptide 1 antibody
    • DEAD box protein 1 antibody
    • DEAD box protein retinoblastoma antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 1 antibody
    see all

Anti-DDX1 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling DDX1 with ab70252 at 1/200 (1µg/ml). Detection: DAB.
  • Immunoprecipitation/ Western Blot of DDX1
    HeLa whole cell lysate, (1 mg for IP, 1/4 of IP loaded) with ab70252 at 0.03µg/ml for WB.
    Lane 1: ab70251 at 3µg/mg lysate for IP.
    Lane 2: Control IgG.

    Detection: Chemiluminescence with exposure time of 1 minute.
  • All lanes : Anti-DDX1 antibody (ab70252) at 0.03 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 20 µg
    Lane 2 : Whole cell lysate from Hela cells at 40 µg
    Lane 3 : Whole cell lysate from Hela cells at 80 µg


    Predicted band size : 83 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 32 kDa,50 kDa,60 kDa. We are unsure as to the identity of these extra bands.

Anti-DDX1 antibody (ab70252)参考文献

ab70252 has not yet been referenced specifically in any publications.

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