概述

  • 产品名称Anti-DDR2抗体
    参阅全部 DDR2 一抗
  • 描述
    兔多克隆抗体to DDR2
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Cow, Dog, Human, Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human DDR2.

    (Peptide available as ab86427.)

  • 阳性对照
    • This antibody gave a positive signal in the following Lysates: PC12 Whole Cell, Rat Liver Tissue, Mouse Pancreas Tissue and FFPE human skin melanoma tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab76967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa). Abcam recommends using 3% milk as the blocking agent.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能This tyrosine kinase receptor for fibrillar collagen mediates fibroblast migration and proliferation. Contributes to cutaneous wound healing.
  • 组织特异性The major 10 kDa transcript is expressed in high levels in heart and lung, less in brain, placenta, liver, skeletal muscle, pancreas, and kidney.
  • 疾病相关Defects in DDR2 are the cause of spondyloepimetaphyseal dysplasia short limb-hand type (SEMD-SL) [MIM:271665]. A bone disease characterized by short-limbed dwarfism, a narrow chest with pectus excavatum, brachydactyly in the hands and feet, a characteristic craniofacial appearance and premature calcifications. The radiological findings are distinctive and comprise short long bones throughout the skeleton with striking epiphyses that are stippled, flattened and fragmented and flared, irregular metaphyses. Platyspondyly in the spine with wide intervertebral spaces is observed and some vertebral bodies are pear-shaped with central humps, anterior protrusions and posterior scalloping.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 1 F5/8 type C domain.
    Contains 1 protein kinase domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD167 antigen-like family member B antibody
    • CD167b antibody
    • CD167b antigen antibody
    • Cell migration inducing protein 20 antibody
    • DDR 2 antibody
    • DDR2 antibody
    • DDR2_HUMAN antibody
    • Discoidin domain containing receptor 2 antibody
    • Discoidin domain receptor 2 antibody
    • Discoidin domain receptor family member 2 antibody
    • discoidin domain receptor tyrosine kinase 2 antibody
    • Discoidin domain-containing receptor 2 antibody
    • discoidin domain-containing receptor tyrosine kinase 2 antibody
    • Hydroxyaryl protein kinase antibody
    • MIG20a antibody
    • Migration inducing gene 16 protein antibody
    • Neurotrophic tyrosine kinase antibody
    • Neurotrophic tyrosine kinase receptor related 3 antibody
    • NTRKR 3 antibody
    • NTRKR3 antibody
    • Receptor protein tyrosine kinase TKT antibody
    • Receptor protein-tyrosine kinase TKT antibody
    • Receptor related 3 antibody
    • receptor-related 3 antibody
    • TKT antibody
    • TYRO 10 antibody
    • TYRO10 antibody
    • Tyrosine kinase receptor related to neurotrophic TRK antibody
    • Tyrosine protein kinase TYRO 10 antibody
    • Tyrosine protein kinase TYRO10 antibody
    • Tyrosine-protein kinase TYRO10 antibody
    • Tyrosylprotein kinase antibody
    see all

Anti-DDR2 antibody 图像

  • All lanes : Anti-DDR2 antibody (ab76967) at 1 µg/ml

    Lane 1 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 2 : Liver (Rat) Tissue Lysate
    Lane 3 : Pancreas (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 97 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)
  • IHC image of DDR2 staining in human heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76967, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-DDR2 antibody (ab76967)参考文献

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Thanks for your email.

I can certainly provide you an Abcam credit so you can use the funds to purchase another product from us in the future. Please see below below for information on the credit number.

Also unfortunately I will no...

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Thanks so much for the additional information.

It is possible that this antibody is not binding DDR2. If you have purchased this antibody within the last 6 months or so I would be happy to offer a replacement, credit or refund. In your reply,...

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Thank you for your enquiry.

I have a few additional questions:

1. Are samples denatured via SDS too

2. How are you reducing samples?

3. What is the extraction procedure and extraction buffer recipe?

4. ...

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Thank you for your call today and for letting us know about the trouble with this antibody. As we discussed, I am sending a free of charge vial of ab82856 on the order *** which should arrive tomorrow. Please keep me updated with how th...

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