Mouse, Rat, Human
Synthetic peptide corresponding to 12 amino acids from near the center of human DDIT4 (NP_061931)
Human kidney tissue lysate; Mouse kidney tissue.
Shipped at 4°C. Store at 4°C (stable for up to 12 months).
Preservative: 0.02% Sodium Azide
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 25 kDa.
Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Promotes neuronal cell death.
Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients (at protein level).
Belongs to the DDIT4 family.
Information by UniProt
Western blot - DDIT4 antibody (ab106356)
Lane 1 : Anti-DDIT4 antibody (ab106356) at 0.5 µg/ml Lane 2 : Anti-DDIT4 antibody (ab106356) at 1 µg/ml All lanes : Human kidney tissue lysate Lysates/proteins at 15 µg per lane. Predicted band size: 25 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DDIT4 antibody (ab106356)
Immunohistochemistry detection of DDIT4 in paraffin embedded mouse kidney tissue section using ab106356 at 5 µg/ml.
Immunocytochemistry/ Immunofluorescence - Anti-DDIT4 antibody (ab106356)
Immunofluorescence of DDIT4 in mouse kidney tissue with ab106356 at 20 μg/mL.
This product has been referenced in:
et al. Ascorbate attenuates pulmonary emphysema by inhibiting tobacco smoke and Rtp801-triggered lung protein modification and proteolysis. Proc Natl Acad Sci U S A 113:E4208-17 (2016).
Read more (PubMed: 27382160) »
et al. DNA Damage-Inducible Transcript 4 Is an Innate Surveillant of Hair Follicular Stress in Vitamin D Receptor Knockout Mice and a Regulator of Wound Re-Epithelialization. Int J Mol Sci 17:N/A (2016).
Read more (PubMed: 27898044) »
See all 3 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"