GADD153 antibody detects endogenous levels of total GADD153 protein.
Synthesized peptide derived from human GADD153.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 19 kDa).
Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA.
Note=A chromosomal aberration involving DDIT3 is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with FUS.
Belongs to the bZIP family.
Contains 1 bZIP domain.
Information by UniProt
- C/EBP homologous protein antibody
- C/EBP Homology Protein antibody
- C/EBP zeta antibody
Western blot - GADD153 antibody (ab53081)
All lanes :
Anti-DDIT3 antibody (ab53081) at 1/500 dilutionLane 1 :
LOVO cell extract (treated with 10% serum for 15 mins.) with no blocking peptideLane 2 :
LOVO cell extract (treated with 10% serum for 15 mins.) with blocking peptide (ab53081 immunogen)Predicted band size :
19 kDaObserved band size :
25 kDa (why is the actual band size different from the predicted?
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