Shipped at 4°C. Store at +4°C. Store In the Dark.
Preservative: 2mM Sodium Azide
Constituents: 0.1% BSA, 50mM Sucrose, 150mM Sodium chloride, 100mM Sodium phosphate, pH 7.4
Concentration information loading...
Purified using size exclusion chromatography as well as some proprietary methods for purifying the final materials.
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use at an assay dependent dilution.
||Use a concentration of 2 µg/ml.
Anti-DDDDK tag antibody [M2] (SureLight® P3) 图像
Western blot - Anti-DDDDK tag antibody [M2] (SureLight® P3) (ab72958)Image part of an Abreview submitted by Antibody Solutions
All lanes :
Anti-DDDDK tag antibody [M2] (SureLight® P3) (ab72958) at 2 µg/mlLane 1 :
HEK293 cell line transfected with empty vectorLane 2 :
HEK293 cell line transfected with vector overexpressing DDDDK tagSecondary
Goat anti-mouse IgG conjugated to HRP at 1/5000 dilution
Performed under reducing conditions.
Image part of an Abreview submitted by Antibody Solutions
Blocking performed for 16 hours/ 4o
C using 3.0% Non-fat milk, PBS, 0.1% Tween-20.
Anti-DDDDK tag antibody [M2] (SureLight® P3) (ab72958)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"