小鼠单克隆抗体[M2] to DDDDK tag (SureLight® P3)
SureLight® P3. Ex: 614nm, Em: 662nm
Synthetic peptide corresponding to DDDDK tag .
Excitation max. 614 nm Emission max. 662 nm
Shipped at 4°C. Store at +4°C. Store In the Dark.
Preservative: 2mM Sodium Azide
Constituents: 0.1% BSA, 50mM Sucrose, 150mM Sodium chloride, 100mM Sodium phosphate, pH 7.4
Concentration information loading...
Purified using size exclusion chromatography as well as some proprietary methods for purifying the final materials.
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use at an assay dependent dilution.
||Use a concentration of 2 µg/ml.
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
Western blot - Anti-DDDDK tag antibody [M2] (SureLight® P3) (ab72958)Image part of an Abreview submitted by Antibody Solutions
All lanes :
Anti-DDDDK tag antibody [M2] (SureLight® P3) (ab72958) at 2 µg/mlLane 1 :
HEK293 cell line transfected with empty vectorLane 2 :
HEK293 cell line transfected with vector overexpressing DDDDK tagSecondaryAll lanes :
Goat anti-mouse IgG conjugated to HRP at 1/5000 dilution
Performed under reducing conditions.
Blocking performed for 16 hours/ 4o
C using 3.0% Non-fat milk, PBS, 0.1% Tween-20.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"