Anti-DDDDK tag抗体[FG4R] - (Equivalent to FLAG抗体from Sigma) (ab125243)

概述

  • 产品名称Anti-DDDDK tag抗体[FG4R] - (Equivalent to FLAG抗体from Sigma)
    参阅全部 DDDDK tag 一抗
  • 描述
    小鼠单克隆抗体[FG4R] to DDDDK tag - (Equivalent to FLAG抗体from Sigma)
  • 特异性Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins.
  • 经测试应用适用于: WB, ELISA, Dot blot, IP, In situ hybridizationmore details
  • 免疫原

    Synthetic peptide corresponding to DDDDK tag conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    DYKDDDDK

  • 阳性对照
    • HEK293 cells transfected with DYKDDDDK
  • 常规说明If stored at 4 °C please add 0.1% sodium azide.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度Ascites
  • 纯化说明ab125243 was purified by protein A affinity chromatography from mouse ascites fluid
  • 克隆单克隆
  • 克隆编号FG4R
  • 同种型IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab125243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/3000. (with ECL)
ELISA Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
In situ hybridization 1/500 - 1/2000.

靶标

  • 相关性This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
  • 别名
    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG antibody
    • FLAG tag antibody
    see all

Anti-DDDDK tag antibody [FG4R] - (Equivalent to FLAG antibodies from Sigma) 图像

  • All lanes : Anti-DDDDK tag antibody [FG4R] - (Equivalent to FLAG antibodies from Sigma) (ab125243) at 1/1000 dilution

    Lane 1 : HEK293 cell lysate non-transfected
    Lane 2 : HEK293 cell lysate transfected with DDDDK tagged protein vector

    Developed using the ECL technique

Anti-DDDDK tag antibody [FG4R] - (Equivalent to FLAG antibodies from Sigma) (ab125243)参考文献

This product has been referenced in:
  • Tang S  et al. RasGRP3 limits Toll-like receptor-triggered inflammatory response in macrophages by activating Rap1 small GTPase. Nat Commun 5:4657 (2014). WB . Read more (PubMed: 25118589) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"