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Synthetic peptide corresponding to DDDDK tag conjugated to Keyhole Limpet Haemocyanin (KLH).
Our Abpromise guarantee covers the use of ab18230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 2 - 10 µg/ml.|
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).|
|ICC/IF||Use at an assay dependent dilution.|
ab18230 staining DDDDK tag in Human HeLa, cervical carcinoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% tween20 and blocked with 0.5% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/200 in PBS) for 24 hours at 4°C. Ab8517 (1/200) was used as the secondary antibody
Image part of an abreview submitted by Antibody Solutions LtdBlocked for 16 hours/ 4oC in 3.0% Non-fat milk, PBS, 0.1% Tween-20
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