Anti-DDDDK tag抗体[F-tag-01] - (Equivalent to FLAG抗体from Sigma) (ab18230)

概述

  • 产品名称
    Anti-DDDDK tag抗体[F-tag-01] - (Equivalent to FLAG抗体from Sigma)
    参阅全部 DDDDK tag 一抗
  • 描述
    小鼠单克隆抗体[F-tag-01] to DDDDK tag - (Equivalent to FLAG抗体from Sigma)
  • 经测试应用
    适用于: ICC, WB, ICC/IFmore details
    不适用于: IP
  • 种属反应性
    Fusion proteins from all species containing the DYKDDDDK epitope.
  • 免疫原

    Synthetic peptide corresponding to DDDDK tag conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    DYKDDDDK

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    单克隆
  • 克隆编号
    F-tag-01
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab18230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use a concentration of 2 - 10 µg/ml.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ICC/IF Use at an assay dependent dilution.
  • 应用说明
    Is unsuitable for IP.
  • 靶标

    • 相关性
      This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
    • 别名
      • DDDDK epitope tag antibody
      • DDDK antibody
      • ddk antibody
      • DYKDDDDK antibody
      • DYKDDDDK epitope tag antibody
      • DYKDDDDK tag antibody
      • ECS epitope tag antibody
      • ECS tag antibody
      • Enterokinase Cleavage Site epitope tag antibody
      • Enterokinase Cleavage Site tag antibody
      • FLAG antibody
      • FLAG tag antibody
      see all

    图片

    • ab18230 staining DDDDK tag in Human HeLa, cervical carcinoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% tween20 and blocked with 0.5% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/200 in PBS) for 24 hours at 4°C. Ab8517 (1/200) was used as the secondary antibody

      See Abreview

    • All lanes : Anti-DDDDK tag antibody [F-tag-01] - (Equivalent to FLAG antibodies from Sigma) (ab18230) at 2 µg/ml

      Lane 1 : Hek293 cells transfected with empty vector
      Lane 2 : HEK293 cells transfected with vector overexpressing DDDDK tag

      Secondary
      Goat anti-mouse IgG conjugated to HRP

      Performed under reducing conditions.

      Predicted band size : 55 kDa

      Image part of an abreview submitted by Antibody Solutions Ltd

      Blocked for 16 hours/ 4oC in 3.0% Non-fat milk, PBS, 0.1% Tween-20

      See Abreview

    • (a) Fusion of nuclear protein stained with ab18230 (red)
      (b) Cell nuclei stained with DAPI (blue)
      (c) Merge of (a) and (b) confirming nuclear localisation of the fusion protein.
    • Immunofluorescence analysis of transfected HEK293 cells, staining DDDDK tag with ab18230 at 1/200 dilution.

    文献

    This product has been referenced in:
    • Dave JM  et al. Hic-5 mediates the initiation of endothelial sprouting by regulating a key surface metalloproteinase. J Cell Sci 129:743-56 (2016). WB . Read more (PubMed: 26769900) »
    • Guo H  et al. The aspirin-induced long non-coding RNA OLA1P2 blocks phosphorylated STAT3 homodimer formation. Genome Biol 17:24 (2016). WB . Read more (PubMed: 26898989) »

    See all 16 Publications for this product

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 22°C
    Sample
    Monkey Cell (COS-7)
    Specification
    COS-7
    Permeabilization
    Yes - 0.2% triton
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Sep 12 2013

    Merci de nous avoir contactés.

    Nous avons plusieurs anti-FLAG anticorps au catalogue par contre aucun n’a été testé en microscopie électronique.

    Cependant, je peux vous proposer :

    a...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Recombinant protein (Rear leg muscle (homogenised))
    Loading amount
    20 µg
    Specification
    Rear leg muscle (homogenised)
    Gel Running Conditions
    Reduced Denaturing (16% Tricine)
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 04 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293 cells transfected with empty vector or FH-FLAG)
    Loading amount
    20 µg
    Specification
    293 cells transfected with empty vector or FH-FLAG
    Gel Running Conditions
    Reduced Denaturing (Pierce precise protein gel 4-20%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Mr. Antibody Solutions

    Verified customer

    提交于 Dec 17 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Escherichia coli Cell lysate - whole cell (chimeric protein)
    Loading amount
    2 µg
    Specification
    chimeric protein
    Gel Running Conditions
    Reduced Denaturing (4-12% bis tris)
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 17 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa, cervical carcinoma)
    Specification
    HeLa, cervical carcinoma
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0,3% Tween20
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 25 2009

    Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab18230 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293 transfected with a 27 kDa construct)
    Loading amount
    25 µg
    Specification
    HEK293 transfected with a 27 kDa construct
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
    Username

    Abcam user community

    Verified customer

    提交于 Apr 18 2007

    Thank you for your enquiry, we do not know if it will recognise the FLAG tag, we know this antibody will recognise recombinant proteins containing the DYKDDDK sequence.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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