Anti-DDDDK tag抗体- (Equivalent to FLAG抗体from Sigma) (HRP) (ab2493)

概述

  • 产品名称
    Anti-DDDDK tag抗体- (Equivalent to FLAG抗体from Sigma) (HRP)
    参阅全部 DDDDK tag 一抗
  • 描述
    兔多克隆抗体to DDDDK tag - (Equivalent to FLAG抗体from Sigma) (HRP)
  • 偶联物
    HRP
  • 经测试应用
    适用于: ELISA, WBmore details
  • 免疫原

    Peptide xxxDDDDK conjugated to KLH. xxx represents random amino acids. This sequence represents the Enterokinase Cleavage Site.

  • 常规说明
    Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, pH 7
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab2493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000 - 1/20000.
WB 1/1000 - 1/10000.

靶标

  • 相关性
    This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
  • 别名
    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG antibody
    • FLAG tag antibody
    see all

实验方案

Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (HRP) (ab2493)参考文献

ab2493 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (yeast cell lysate)
Specification
yeast cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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提交于 Mar 26 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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