Anti-DDDDK tag抗体- (Equivalent to FLAG抗体from Sigma) (HRP) (ab2491)

概述

  • 产品名称Anti-DDDDK tag抗体- (Equivalent to FLAG抗体from Sigma) (HRP)
    参阅全部 DDDDK tag 一抗
  • 描述
    鸡多克隆抗体to DDDDK tag - (Equivalent to FLAG抗体from Sigma) (HRP)
  • 偶联物HRP
  • 经测试应用适用于: WB, ELISAmore details
  • 免疫原

    Peptide

    xxxDDDDK

    conjugated to KLH. xxx represents random amino acids. This sequence represents the Enterokinase Cleavage Site.

  • 常规说明Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液50 mM HEPES pH 7.0, 0.1 M NaCl, 0.2% BSA, 0.01% Thimerosal
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Antibodies were affinity purified using the peptide coupled to a different carrier.
  • 克隆多克隆
  • 同种型IgY
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab2491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明The affinity purified antibodies were analyzed by ELISA and Western Blot analyses.
    In a direct ELISA against 1 ug of ECS peptide coupled to BSA as sample, conjugate diluted 1:35,000 produced an O.D.=1.0 in a 15 minute reaction using TMB as substrate.
    Optimal conjugate working dilutions should be determined for your particular assay conditions,
  • 靶标

    • 相关性This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
    • 别名
      • DDDDK epitope tag antibody
      • DDDK antibody
      • ddk antibody
      • DYKDDDDK antibody
      • DYKDDDDK epitope tag antibody
      • DYKDDDDK tag antibody
      • ECS epitope tag antibody
      • ECS tag antibody
      • Enterokinase Cleavage Site epitope tag antibody
      • Enterokinase Cleavage Site tag antibody
      • FLAG antibody
      • FLAG tag antibody
      see all

    Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (HRP) (ab2491)参考文献

    ab2491 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"