Anti-DDDDK tag抗体- (Equivalent to FLAG抗体from Sigma) (HRP) (ab1238)

概述

  • 产品名称Anti-DDDDK tag抗体- (Equivalent to FLAG抗体from Sigma) (HRP)
    参阅全部 DDDDK tag 一抗
  • 描述
    山羊多克隆抗体to DDDDK tag - (Equivalent to FLAG抗体from Sigma) (HRP)
  • 偶联物HRP
  • 经测试应用适用于: ELISA, WB, ICCmore details
  • 免疫原

    Full length native protein (purified) corresponding to DDDDK tag conjugated to Keyhole Limpet Haemocyanin (KLH).

  • 常规说明Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: None
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, pH 7
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab1238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000 - 1/100000.
WB 1/1000 - 1/30000.
ICC 1/200 - 1/500.

靶标

  • 相关性This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
  • 别名
    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG antibody
    • FLAG tag antibody
    see all

Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (HRP) 图像

  • Anti-Flag immunostaining (using ab1238) of ntl:Flag BAC transgenic zebrafish embryos shows expression in the margin at 6 hours post fertilisation.

Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (HRP) (ab1238)参考文献

This product has been referenced in:
  • Yee A  et al. A von Willebrand factor fragment containing the D'D3 domains is sufficient to stabilize coagulation factor VIII in mice. Blood 124:445-52 (2014). Read more (PubMed: 24850761) »
  • Biffi G  et al. Quantitative visualization of DNA G-quadruplex structures in human cells. Nat Chem 5:182-6 (2013). Read more (PubMed: 23422559) »

See all 6 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"