Anti-DDDDK tag抗体- (Equivalent to FLAG抗体from Sigma) (ab1170)

概述

  • 产品名称Anti-DDDDK tag抗体- (Equivalent to FLAG抗体from Sigma)
    参阅全部 DDDDK tag 一抗
  • 描述
    鸡多克隆抗体to DDDDK tag - (Equivalent to FLAG抗体from Sigma)
  • 经测试应用适用于: ELISA, WB, ICC/IFmore details
  • 免疫原

    Peptide

    xxxDDDDK

    conjugated to KLH. xxx represents random amino acids. This sequence represents the Enterokinase Cleavage Site.

  • 常规说明Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Antibodies were affinity purified using the peptide coupled to a different carrier.
  • 克隆多克隆
  • 同种型IgY
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab1170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000 - 1/20000.
WB 1/2000 - 1/10000.
ICC/IF Use at an assay dependent concentration.

靶标

  • 相关性This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
  • 别名
    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG antibody
    • FLAG tag antibody
    see all

Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) 图像

  • ab1170 staining DDDDK in Mouse primary hippocampal neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in 1x PBS + 5% Goat serum + 0.1% Triton-X 100) for 24 hours at 4°C. An Alexa Fluor® 555-conjugated Goat anti-chicken IgY polyclonal (1/400) was used as the secondary antibody.

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  • Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (ab1170) at 1/5000 dilution

    Secondary
    Rabbit Anti-Chicken IgY H&L (HRP) (ab6753) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 66 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

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Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (ab1170)参考文献

ab1170 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Mouse Cell (Primary hippocampal neurons)
Specification Primary hippocampal neurons
Permeabilization No
Fixative Paraformaldehyde
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提交于 Oct 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Escherichia coli Recombinant protein (BL21)
Loading amount 0.1 µg
Specification BL21
Gel Running Conditions Reduced Denaturing (4~20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Sep 30 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Escherichia coli Purified protein (Mach1cell)
Loading amount 0.5 µg
Specification Mach1cell
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Sep 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"