概述

  • 产品名称Anti-DDB1抗体
    参阅全部 DDB1 一抗
  • 描述
    兔多克隆抗体to DDB1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH, from the C terminal of Human DDB1 (NP_001914.3).

  • 阳性对照
    • Human Brain Cortex tissue.

性能

应用

Our Abpromise guarantee covers the use of ab117254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 127 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the DDB1 family.
  • 翻译后修饰Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis.
  • 细胞定位Cytoplasm. Nucleus. Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Damage specific DNA binding protein 1 antibody
    • Damage-specific DNA-binding protein 1 antibody
    • DDB 1 antibody
    • DDB p127 subunit antibody
    • Ddb1 antibody
    • DDB1_HUMAN antibody
    • DDBa antibody
    • DNA damage binding protein 1 antibody
    • DNA damage-binding protein 1 antibody
    • DNA damage-binding protein a antibody
    • HBV X-associated protein 1 antibody
    • UV damaged DNA binding factor antibody
    • UV damaged DNA binding protein 1 antibody
    • UV DDB 1 antibody
    • UV DDB1 antibody
    • UV-damaged DNA-binding factor antibody
    • UV-damaged DNA-binding protein 1 antibody
    • UV-DDB 1 antibody
    • X associated protein 1 antibody
    • XAP 1 antibody
    • XAP-1 antibody
    • XAP1 antibody
    • Xeroderma pigmentosum group E complementing protein antibody
    • Xeroderma pigmentosum group E-complementing protein antibody
    • XPCe antibody
    • XPE antibody
    • XPE BF antibody
    • XPE binding factor antibody
    • XPE-BF antibody
    • XPE-binding factor antibody
    see all

Anti-DDB1 antibody 图像

  • ab117254, at 5 µg/ml, staining DDB1 in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-DDB1 antibody (ab117254)参考文献

ab117254 has not yet been referenced specifically in any publications.

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