Anti-DDAH2抗体(ab232694)
Key features and details
- Rabbit polyclonal to DDAH2
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
概述
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产品名称
Anti-DDAH2抗体
参阅全部 DDAH2 一抗 -
描述
兔多克隆抗体to DDAH2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human, Pig
预测可用于: Mouse, Rat, Cow -
免疫原
Recombinant fragment (His-tag) corresponding to Human DDAH2 aa 1 to the C-terminus. (Expressed in E.coli).
Database link: O95865 -
阳性对照
- WB: Recombinant human DDAH2 protein; Pig brain lysate; MCF7 cell lysate. IHC-P: Human prostate, brain, colorectal cancer, glioma and stomach cancer tissues.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab232694 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab232694于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 30 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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说明 |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 30 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
靶标
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功能
Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. -
组织特异性
Detected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain. -
序列相似性
Belongs to the DDAH family. - Information by UniProt
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数据库链接
- Entrez Gene: 540386 Cow
- Entrez Gene: 23564 Human
- Entrez Gene: 51793 Mouse
- Entrez Gene: 294239 Rat
- Omim: 604744 Human
- SwissProt: Q3SX44 Cow
- SwissProt: O95865 Human
- SwissProt: Q99LD8 Mouse
see all -
别名
- DDAH antibody
- DDAH II antibody
- DDAH-2 antibody
see all
图片
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Paraffin-embedded human prostate tissue stained for DDAH2 using ab232694 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human brain tissue stained for DDAH2 using ab232694 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Anti-DDAH2 antibody (ab232694) at 2 µg/ml + Recombinant human DDAH2 protein
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 30 kDa -
All lanes : Anti-DDAH2 antibody (ab232694) at 2 µg/ml
Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 2 : Pig brain lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 30 kDa -
Paraffin-embedded human colorectal cancer tissue stained for DDAH2 using ab232694 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human glioma tissue stained for DDAH2 using ab232694 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human stomach cancer tissue stained for DDAH2 using ab232694 at 20 μg/ml in immunohistochemical analysis. DAB staining.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab232694 被引用在 1 文献中.
- Hannemann J et al. Upregulation of DDAH2 Limits Pulmonary Hypertension and Right Ventricular Hypertrophy During Chronic Hypoxia in Ddah1 Knockout Mice. Front Physiol 11:597559 (2020). PubMed: 33281630