概述

  • 产品名称
  • 描述
    山羊多克隆抗体to DDAH1
  • 经测试应用
    适用于: IHC-P, ICC/IF, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Cow
  • 免疫原

    Synthetic peptide:

    TCCSVLINKKVDS

    , corresponding to C terminal amino acids 272-284 of Human DDAH1.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab2231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 24663083
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).Can be blocked with Human DDAH1 peptide (ab99047).

靶标

  • 功能
    Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
  • 组织特异性
    Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle.
  • 序列相似性
    Belongs to the DDAH family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DDAH antibody
    • DDAH I antibody
    • DDAH-1 antibody
    • DDAH1 antibody
    • DDAH1_HUMAN antibody
    • DDAHI antibody
    • Dimethylargininase 1 antibody
    • Dimethylargininase-1 antibody
    • Dimethylarginine dimethylaminohydrolase 1 antibody
    • N(G) antibody
    • N(G)-dimethylarginine dimethylaminohydrolase 1 antibody
    • NG NG dimethylarginine dimethylaminohydrolase antibody
    see all

图片

  • Anti-DDAH1 antibody (ab2231) at 0.10000000149 µg/ml + Human kidney lysate at 35 µg

    Predicted band size : 31 kDa
  • ICC/IF image of ab2231 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2231, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab2231 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2231, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

文献

This product has been referenced in:
  • D'Mello R  et al. Dimethylarginine dimethylaminohydrolase 1 is involved in spinal nociceptive plasticity. Pain 156:2052-60 (2015). WB, IHC-FoFr ; Rat . Read more (PubMed: 26098438) »
  • Prins JM  et al. Cd²?-induced alteration of the global proteome of human skin fibroblast cells. J Proteome Res 13:1677-87 (2014). WB . Read more (PubMed: 24527689) »

See all 9 Publications for this product

客户评价及客户问答

Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain (Hippocampus))
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
Brain (Hippocampus)
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

圭一 角山

Verified customer

提交于 Nov 25 2015

Application
Western blot
Sample
Mouse Tissue lysate - whole (hippocampus)
Loading amount
5 µg
Specification
hippocampus
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 09 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Hippocampus lysate)
Loading amount
40 µg
Specification
Hippocampus lysate
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Richard D'Mello

Verified customer

提交于 Apr 14 2009

According to the datasheet, this antibody is thought to run at 40kDa due glycosylation. It could be that the protein is running at 32kDa in their experiments as the protein in their system is not glycosylated. However, I am concerned that their negativ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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