Anti-DCXR抗体[4G4AF5] (ab110283)
Key features and details
- Mouse monoclonal [4G4AF5] to DCXR
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-DCXR抗体[4G4AF5]
参阅全部 DCXR 一抗 -
描述
小鼠单克隆抗体[4G4AF5] to DCXR -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IF, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus. This information is considered to be commercially sensitive.
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阳性对照
- Isolated human liver mitochondria; Human HDFn cells; HL-60 cells.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by affinity purification. Purity near homogeneity as judged by SDS-PAGE. Purity >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
4G4AF5 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110283于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.
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ICC/IF |
Use a concentration of 4 µg/ml.
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa. |
ICC/IF
Use a concentration of 4 µg/ml. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Catalyzes the NADPH-dependent reduction of several pentoses, tetroses, trioses, alpha-dicarbonyl compounds and L-xylulose. Participates in the uronate cycle of glucose metabolism. May play a role in the water absorption and cellular osmoregulation in the proximal renal tubules by producing xylitol, an osmolyte, thereby preventing osmolytic stress from occurring in the renal tubules. -
组织特异性
Highly expressed in kidney, liver and epididymis. In the epididymis, it is mainly expressed in the proximal and distal sections of the corpus region. Weakly or not expressed in brain, lung, heart, spleen and testis. -
疾病相关
Note=The enzyme defect in pentosuria has been shown to involve L-xylulose reductase. Essential pentosuria is an inborn error of metabolism characterized by the excessive urinary excretion of the pentose L-xylulose. -
序列相似性
Belongs to the short-chain dehydrogenases/reductases (SDR) family. -
细胞定位
Membrane. Probably recruited to membranes via an interaction with phosphatidylinositol. - Information by UniProt
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数据库链接
- Entrez Gene: 51181 Human
- Omim: 608347 Human
- SwissProt: Q7Z4W1 Human
- Unigene: 9857 Human
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别名
- Carbonyl reductase 2 antibody
- Carbonyl reductase II antibody
- DCR antibody
see all
图片
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Anti-DCXR antibody [4G4AF5] (ab110283) at 1 µg/ml + Human liver mitochondria at 20 µg
Secondary
Goat anti-mouse conjugated to AP
Predicted band size: 26 kDa -
Immunocytochemistry image of ab110283 stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with ab110283 at 4 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (green) AlexaFluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in the cell membrane.
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HL-60 cells were stained with 1 µg/mL ab110283 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab110283 被引用在 2 文献中.
- Murdica V et al. In vitro cultured human endometrial cells release extracellular vesicles that can be uptaken by spermatozoa. Sci Rep 10:8856 (2020). PubMed: 32483153
- Murdica V et al. Seminal plasma of men with severe asthenozoospermia contain exosomes that affect spermatozoa motility and capacitation. Fertil Steril 111:897-908.e2 (2019). PubMed: 31029245