Anti-DCXR抗体[4G4AF5] (ab110283)

概述

  • 产品名称Anti-DCXR抗体[4G4AF5]
    参阅全部 DCXR 一抗
  • 描述
    小鼠单克隆抗体[4G4AF5] to DCXR
  • 经测试应用适用于: WB, ICC/IF, In-Cell ELISA, Flow Cyt, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human liver mitochondria

  • 阳性对照
    • Isolated human liver mitochondria; Human HDFn cells; HL-60 cells.
  • 常规说明

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

Our Abpromise guarantee covers the use of ab110283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.
ICC/IF Use a concentration of 4 µg/ml.
In-Cell ELISA Use a concentration of 4 µg/ml. (0.4 µg/well).
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.

靶标

  • 功能Catalyzes the NADPH-dependent reduction of several pentoses, tetroses, trioses, alpha-dicarbonyl compounds and L-xylulose. Participates in the uronate cycle of glucose metabolism. May play a role in the water absorption and cellular osmoregulation in the proximal renal tubules by producing xylitol, an osmolyte, thereby preventing osmolytic stress from occurring in the renal tubules.
  • 组织特异性Highly expressed in kidney, liver and epididymis. In the epididymis, it is mainly expressed in the proximal and distal sections of the corpus region. Weakly or not expressed in brain, lung, heart, spleen and testis.
  • 疾病相关Note=The enzyme defect in pentosuria has been shown to involve L-xylulose reductase. Essential pentosuria is an inborn error of metabolism characterized by the excessive urinary excretion of the pentose L-xylulose.
  • 序列相似性Belongs to the short-chain dehydrogenases/reductases (SDR) family.
  • 细胞定位Membrane. Probably recruited to membranes via an interaction with phosphatidylinositol.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Carbonyl reductase 2 antibody
    • Carbonyl reductase II antibody
    • DCR antibody
    • Dcxr antibody
    • DCXR_HUMAN antibody
    • Dicarbonyl L xylulose reductase antibody
    • Dicarbonyl/ L xylulose reductase antibody
    • Dicarbonyl/L-xylulose reductase antibody
    • HCR2 antibody
    • HCRII antibody
    • Human carbonyl reductase 2 antibody
    • kiDCR antibody
    • Kidney dicarbonyl reductase antibody
    • L xylulose reductase antibody
    • L-xylulose reductase antibody
    • P34H antibody
    • PNTSU antibody
    • SDR20C1 antibody
    • Short chain dehydrogenase/reductase family 20C member 1 antibody
    • Sperm surface protein P34H antibody
    • XR antibody
    see all

Anti-DCXR antibody [4G4AF5] 图像

  • Anti-DCXR antibody [4G4AF5] (ab110283) at 1 µg/ml + Human liver mitochondria at 20 µg

    Secondary
    Goat anti-mouse conjugated to AP

    Predicted band size : 26 kDa
  • Immunocytochemistry image of ab110283 stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with ab110283 at 4 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (green) AlexaFluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in the cell membrane.
  • HL-60 cells were stained with 1 µg/mL ab110283 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

Anti-DCXR antibody [4G4AF5] (ab110283)参考文献

ab110283 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab110283.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"