Synthetic peptide:
C-RPQIAGTKSTT
, corresponding to C terminal amino acids 248-258 of Human DCUN1D1 (NP_065691.2).Our Abpromise guarantee covers the use of ab99506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
WB | Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 30 kDa. |
ab99506 (5µg/ml) staining of paraffin embedded Human Kidney shows pixulated staining in the cytoplasm of epithelial cells of PCT, but heavy cytoplasm staining in the epithelial cells of the DCT. Tissue treated by steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ab99506 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"