概述
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产品名称Anti-DCUN1D1抗体
参阅全部 DCUN1D1 一抗 -
描述山羊多克隆抗体to DCUN1D1
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经测试应用适用于: IHC-P, WBmore details
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种属反应性与反应: Human
预测可用于: Mouse, Cow
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免疫原
Synthetic peptide:
C-RPQIAGTKSTT
, corresponding to C terminal amino acids 248-258 of Human DCUN1D1 (NP_065691.2). -
阳性对照
- HepG2 cell lysate
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab99506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 30 kDa. |
靶标
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相关性DCUN1D1 (DCUN1 domain-containing protein 1) contains a DUF298 domain and a UBA-like ubiquitin. Forms part of an E3 ubiquitin ligase complex for neddylation. Required for neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes by enhancing the rate of cullins neddylation. Functions to recruit the NEDD8-charged E2 enzyme to the cullin component. Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity. Acts also as an oncogene facilitating malignant transformation and carcinogenic progression. Defects in DCUN1D1 may be a cause of squamous cell carcinomas. Strongly overexpressed in thyroid tumors, bronchioloalveolar carcinomas, and malignant tissues of squamous cell carcinoma of the oral tongue. Not overexpressed in aggressive adrenocortical carcinomas.
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数据库链接
- Entrez Gene: 539869 Cow
- Entrez Gene: 54165 Human
- Entrez Gene: 114893 Mouse
- Omim: 605905 Human
- SwissProt: Q96GG9 Human
- SwissProt: Q9QZ73 Mouse
- Unigene: 104613 Human
- Unigene: 374810 Mouse
see all -
别名
- DCN1 defective in cullin neddylation 1 domain containing 1 antibody
- DCN1 like protein 1 antibody
- DCNL1 antibody
see all
Anti-DCUN1D1 antibody 图像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCUN1D1 antibody (ab99506)ab99506 (5µg/ml) staining of paraffin embedded Human Kidney shows pixulated staining in the cytoplasm of epithelial cells of PCT, but heavy cytoplasm staining in the epithelial cells of the DCT. Tissue treated by steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Western blot - DCUN1D1 antibody (ab99506)Anti-DCUN1D1 antibody (ab99506) at 0.5 µg/ml + HepG2 cell lysate at 35 µg
Developed using the ECL technique
Predicted band size : 30 kDa
Exposure time : 1 hour
Anti-DCUN1D1 antibody (ab99506)参考文献
ab99506 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"