重组Anti-DCTN4抗体[EPR11223] (ab170107)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11223] to DCTN4
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-DCTN4抗体[EPR11223]
参阅全部 DCTN4 一抗 -
描述
兔单克隆抗体[EPR11223] to DCTN4 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Recombinant fragment corresponding to Human DCTN4.
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阳性对照
- Human fetal brain, U87-MG, K562 or HeLa lysate, Human kidney tissue, HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR11223 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab170107于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (3) |
1/1000 - 1/10000. Predicted molecular weight: 62 kDa.
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ICC/IF | (1) |
1/100 - 1/250.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use of an HRP/AP polymerized secondary antibody recommended.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 62 kDa. |
ICC/IF
1/100 - 1/250. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use of an HRP/AP polymerized secondary antibody recommended. |
靶标
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功能
Could have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in linking dynein and dynactin to the cortical cytoskeleton. -
序列相似性
Belongs to the dynactin subunit 4 family. -
细胞定位
Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Has a punctate cytoplasmic distribution as well as centrosomal distribution typical of dynactin. Overexpression does not disrupt microtubule organization or the integrity of the Golgi but does cause both cytosolic and nuclear distribution, suggesting that this polypeptide may be targeted to the nucleus at very high expression levels. - Information by UniProt
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数据库链接
- Entrez Gene: 51164 Human
- Entrez Gene: 67665 Mouse
- Entrez Gene: 84428 Rat
- SwissProt: Q9UJW0 Human
- SwissProt: Q8CBY8 Mouse
- SwissProt: Q9QUR2 Rat
- Unigene: 675564 Human
- Unigene: 272801 Mouse
see all -
别名
- DCTN 4 antibody
- Dctn4 antibody
- DCTN4_HUMAN antibody
see all
图片
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All lanes : Anti-DCTN4 antibody [EPR11223] (ab170107) at 1/1000 dilution
Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 62 kDa -
Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling DCTN4 with ab170107 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescence analysis of HeLa cells labeling DCTN4 with ab170107 at a 1/100 dilution (red) plus Dapi staining (blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab170107 尚未被引用在任何文献中。