重组Anti-DCTN3抗体[EPR5097] (ab124674)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5097] to DCTN3
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-DCTN3抗体[EPR5097]
参阅全部 DCTN3 一抗 -
描述
兔单克隆抗体[EPR5097] to DCTN3 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human DCTN3 aa 100-200. The exact sequence is proprietary.
Database link: O75935 -
阳性对照
- 293T, HeLa, BxPC 3, SH-SY5Y and mouse and rat muscle lysates; Human breast tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 5.58 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5097 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124674于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | (1) |
1/2000 - 1/10000.
|
WB | (4) |
1/2000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
|
IHC-P |
1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
|
说明 |
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ICC/IF
1/2000 - 1/10000. |
WB
1/2000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
IHC-P
1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
靶标
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功能
Together with dynein may be involved in spindle assembly and cytokinesis. -
组织特异性
Ubiquitously expressed. Highly expressed in muscle and pancreas and detected at lower levels in brain. -
序列相似性
Belongs to the dynactin subunit 3 family. -
细胞定位
Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Cleavage furrow. Midbody. Localizes to punctate cytoplasmic structures and to the centrosome during interphase, and to kinetochores and to spindle poles throughout mitosis. Colocalizes with dynein to the cleavage furrow and to midbody of dividing cells. - Information by UniProt
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数据库链接
- Entrez Gene: 11258 Human
- Entrez Gene: 53598 Mouse
- Entrez Gene: 362504 Rat
- Omim: 607387 Human
- SwissProt: O75935 Human
- SwissProt: Q9Z0Y1 Mouse
- Unigene: 511768 Human
- Unigene: 29025 Mouse
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别名
- DCNT22 antibody
- DCTN 22 antibody
- DCTN22 antibody
see all
图片
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All lanes : Anti-DCTN3 antibody [EPR5097] (ab124674) at 1/10000 dilution (purified)
Lane 1 : 293T cell lysate
Lane 2 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Immunohistochemical staining of DCTN3 in paraffin embedded human breast tissue by unpurified ab124674 at 1/100 dilution.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Immunohistochemical staining of paraffin embedded human pancreas with purified ab124674 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunofluorescence staining of BxPC-3 cells with purified ab124674 at a working dilution of 1 in 100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, purified ab124674 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab15120) at a dilution of 1/500.
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Anti-DCTN3 antibody [EPR5097] (ab124674) at 1/10000 dilution (purified) + Rat muscle at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Anti-DCTN3 antibody [EPR5097] (ab124674) at 1/2000 dilution (purified) + Mouse muscle at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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All lanes : Anti-DCTN3 antibody [EPR5097] (ab124674) at 1/1000 dilution (unpurified)
Lane 1 : 293T cell lysates
Lane 2 : Mouse muscle lysates
Lane 3 : HeLa cell lysates
Lane 4 : BxPC 3 cell lysates
Lane 5 : SH-SY5Y cell lysates
Lane 6 : Rat muscle lysates
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-3 & 5-6 : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 21 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab124674 尚未被引用在任何文献中。