概述

  • 产品名称Anti-DCTN3抗体
    参阅全部 DCTN3 一抗
  • 描述
    山羊多克隆抗体to DCTN3
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    C-QVALGNISSKRE

    , corresponding to internal sequence amino acids 41-52 of Human DCTN3 (NP_009165.1).

  • 阳性对照
    • Human kidney lysate

性能

应用

Our Abpromise guarantee covers the use of ab109551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa).

靶标

  • 功能Together with dynein may be involved in spindle assembly and cytokinesis.
  • 组织特异性Ubiquitously expressed. Highly expressed in muscle and pancreas and detected at lower levels in brain.
  • 序列相似性Belongs to the dynactin subunit 3 family.
  • 细胞定位Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Cleavage furrow. Midbody. Localizes to punctate cytoplasmic structures and to the centrosome during interphase, and to kinetochores and to spindle poles throughout mitosis. Colocalizes with dynein to the cleavage furrow and to midbody of dividing cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DCNT22 antibody
    • DCTN 22 antibody
    • DCTN22 antibody
    • Dctn3 antibody
    • DCTN3_HUMAN antibody
    • dynactin 3 (p22) antibody
    • dynactin 3 antibody
    • dynactin 3 isoform 1 antibody
    • Dynactin complex subunit 22 kDa subunit antibody
    • dynactin light chain antibody
    • Dynactin subunit 3 antibody
    • p22 antibody
    see all

Anti-DCTN3 antibody 图像

  • All lanes : Anti-DCTN3 antibody (ab109551) at 0.20000000298 µg/ml

    Lane 1 : Mouse skeletal muscle lysate
    Lane 2 : Rat skeletal muslce lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 21 kDa
  • Anti-DCTN3 antibody (ab109551) at 0.03 µg/ml + Human kidney lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 21 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour

Anti-DCTN3 antibody (ab109551)参考文献

ab109551 has not yet been referenced specifically in any publications.

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