概述

  • 产品名称Anti-DCTN1抗体
    参阅全部 DCTN1 一抗
  • 描述
    山羊多克隆抗体to DCTN1
  • 特异性This antibody is expected to recognise both human isoforms.
  • 经测试应用适用于: IHC-P, ICC, ICC/IF, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Drosophila melanogaster
  • 免疫原

    Synthetic peptide: QEQLHQLHSRLIS, corresponding to amino acids 1266-1278 of Human DCTN1.

  • 阳性对照
    • A549 and Human Testis lysates.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab11806 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent dilution. PubMed: 17932487
IHC-Fr Use at an assay dependent dilution. PubMed: 17932487
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 150 kDa.Can be blocked with Human DCTN1 peptide (ab23214). Approx 150kDa band observed in A549 and Human Testis lysates

靶标

  • 功能Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles.
  • 组织特异性Brain.
  • 疾病相关Defects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B) [MIM:607641]; also known as progressive lower motor neuron disease (PLMND). HMN7B is a neuromuscular disorder. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective degeneration of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
    Defects in DCTN1 are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
    Defects in DCTN1 are the cause of Perry syndrome (PERRYS) [MIM:168605]; also called parkinsonism with alveolar hypoventilation and mental depression. Perry syndrome is a neuropsychiatric disorder characterized by mental depression not responsive to antidepressant drugs or electroconvulsive therapy, sleep disturbances, exhaustion and marked weight loss. Parkinsonism develops later and respiratory failure occurred terminally.
  • 序列相似性Belongs to the dynactin 150 kDa subunit family.
    Contains 1 CAP-Gly domain.
  • 翻译后修饰Ubiquitinated by a SCF complex containing FBXL5, leading to its degradation by the proteasome.
  • 细胞定位Cytoplasm. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • 150 kDa dynein associated polypeptide antibody
    • 150 kDa dynein-associated polypeptide antibody
    • DAP 150 antibody
    • DAP-150 antibody
    • DAP150 antibody
    • DCTN 1 antibody
    • DCTN1 antibody
    • DCTN1_HUMAN antibody
    • DP 150 antibody
    • DP-150 antibody
    • DP150 antibody
    • Dynactin 1 (p150 Glued (Drosophila) homolog) antibody
    • Dynactin 1 (p150 glued homolog Drosophila) antibody
    • Dynactin 1 antibody
    • Dynactin subunit 1 antibody
    • Dynactin1 antibody
    • HMN7B antibody
    • p135 antibody
    • p150 Glued (Drosophila) homolog antibody
    • p150 glued antibody
    • p150 glued homolog antibody
    • p150(GLUED) DROSOPHILA HOMOLOG OF antibody
    • p150-glued antibody
    • p150glued antibody
    see all

Anti-DCTN1 antibody 图像

  • Anti-DCTN1 antibody (ab11806) at 1 µg/ml + Human testis tissue lysate at 35 µg

    Predicted band size : 150 kDa


    Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • ab11806 at 2.5ug/ml staining DCTN1 in human cerebellum tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

  • ab11806 staining DCTN1 in Human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized with 0.5% Triton X and blocked with 3% BSA for 1 hour at 23°C. Samples were incubated with primary antibody (1/100) for 1 hour. An Alexa Fluor®488-conjugated Donkey anti-goat polyclonal (1/2000) was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of murine colon tissue, staining DCTN1 with ab11806.

    Tissue was fixed with paraformaldehyde, permeabilized with 0.01% Triton X-100 and blocked with 5% BSA for 1 hour at 23°C. Samples were incubated with primary antibody (1/200 in diluent) for 12 hours at 4°C. An AlexaFluor®488-conjugated donkey anti-goat polyclonal IgG (1/800) was used as the secondary antibody.

    See Abreview

Anti-DCTN1 antibody (ab11806)参考文献

This product has been referenced in:
  • Chanduri M  et al. Inositol hexakisphosphate kinase 1 (IP6K1) activity is required for cytoplasmic dynein-driven transport. Biochem J 473:3031-47 (2016). Read more (PubMed: 27474409) »
  • Roher AE  et al. Subjects harboring presenilin familial Alzheimer's disease mutations exhibit diverse white matter biochemistry alterations. Am J Neurodegener Dis 2:187-207 (2013). Read more (PubMed: 24093083) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Colon)
Specification Colon
Fixative Paraformaldehyde
Permeabilization Yes - .01% triton
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Nov 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Intestine)
Specification Intestine
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization No
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Aug 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (MEF)
Specification MEF
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X
Username

Abcam user community

Verified customer

提交于 Mar 17 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - other (MEF)
Loading amount 50 µg
Specification MEF
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Feb 22 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (HELA)
Loading amount 50 µg
Specification HELA
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Feb 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (HELA)
Specification HELA
Fixative Methanol
Permeabilization Yes - 0.5% TRITON-x
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Feb 11 2011

Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with zebrafish has not yet been tested. Should you decide to go ahead and purchase this product, pleas...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"