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Our Abpromise guarantee covers the use of ab11806 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent dilution. PubMed: 17932487|
|IHC-Fr||Use at an assay dependent dilution. PubMed: 17932487|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 150 kDa.Can be blocked with Human DCTN1 peptide (ab23214). Approx 150kDa band observed in A549 and Human Testis lysates|
ab11806 at 2.5ug/ml staining DCTN1 in human cerebellum tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.