重组Anti-DcR2抗体[EPR3588(2)] (ab108421)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3588(2)] to DcR2
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-DcR2抗体[EPR3588(2)]
参阅全部 DcR2 一抗 -
描述
兔单克隆抗体[EPR3588(2)] to DcR2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human kidney, Fetal liver, Fetal kidney and Human prostate lysates. IHC-P: human thyroid carcinoma, kidney and tonsil tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3588(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108421于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
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IHC-P |
1/8000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For unpurified use at 1/250 - 1/500. |
说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa. |
IHC-P
1/8000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For unpurified use at 1/250 - 1/500. |
靶标
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功能
Receptor for the cytotoxic ligand TRAIL. Contains a truncated death domain and hence is not capable of inducing apoptosis but protects against TRAIL-mediated apoptosis. Reports are contradictory with regards to its ability to induce the NF-kappa-B pathway. According to PubMed:9382840, it cannot but according to PubMed:9430226, it can induce the NF-kappa-B pathway. -
组织特异性
Widely expressed, in particular in fetal kidney, lung and liver, and in adult testis and liver. Also expressed in peripheral blood leukocytes, colon and small intestine, ovary, prostate, thymus, spleen, pancreas, kidney, lung, placenta and heart. -
序列相似性
Contains 1 death domain.
Contains 3 TNFR-Cys repeats. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 8793 Human
- Omim: 603614 Human
- SwissProt: Q9UBN6 Human
- Unigene: 213467 Human
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别名
- CD 264 antibody
- CD264 antibody
- DcR 2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling DcR2 with purified ab108421 at 1:8000 dilution (0.03 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Anti-DcR2 antibody [EPR3588(2)] (ab108421) at 1/1000 dilution (Purified) + Human kidney lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution
Predicted band size: 42 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?The molecular weight observed is consistent with what has been described in PMID: 12865931
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All lanes : Anti-DcR2 antibody [EPR3588(2)] (ab108421) at 1/1000 dilution (Unpurified)
Lane 1 : Fetal liver lysate
Lane 2 : Fetal kidney lysate
Lane 3 : Human prostate lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 42 kDa -
Unpurified ab108421, at 1/250 dilution, staining DcR2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Unpurified ab108421, at 1/250 dilution, staining DcR2 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab108421 被引用在 1 文献中.
- Bird TG et al. TGFß inhibition restores a regenerative response in acute liver injury by suppressing paracrine senescence. Sci Transl Med 10:N/A (2018). PubMed: 30111642