<0.2% cross-reactivity to TRAIL R2 peptide of the same region.
Not likely to bind TRAIL R1 due to low sequence homology in this region.
May bind TRAIL R4.
May cross react to mouse TRAIL R3 as antibody binds to a similar size protein from mouse spleen on Westerns.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
DcR1 / TRAIL-R3 / TRID / LIT is one of the two putative decoy receptors identified for TRAIL, one of the members of TNF family of apoptosis inducing proteins. The other putative decoy receptor is called DcR2 / TRUNDD / TRAIL-R4. DcR1 is attached to the cell surface through glycophospholipid anchor. It has the extracellular TRAIL binding domain but lacks the cytoplasmic domain to induce apoptotic signal. Hence overexpression of DcR1 inhibits the TRAIL induced apoptosis.
Tumor necrosis factor receptor superfamily member 10C antibody
Tumor necrosis factor receptor superfamily, member 10c, decoy without an intracellular domain antibody
Western blot - Anti-DcR1 antibody (ab1674)
ab1674 recognizes a 66kDa band of expected size in Lane 1 containing TRAIL-3R-Fc protein and showed minimal or no crossreactivity to TRAIL-2R-Fc protein in Lane 2. ab1674 recognizes a 66kDa band of expected size in Lane 1 containing TRAIL-3R-Fc protein and showed minimal or no crossreactivity to TRAIL-2R-Fc protein in Lane 2.