概述

  • 产品名称Anti-Dcp2抗体
    参阅全部 Dcp2 一抗
  • 描述
    兔多克隆抗体to Dcp2
  • 经测试应用适用于: ICC/IF, WB, IHC-P, EMSA, IPmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Dcp2.

    (Peptide available as ab30458.)

  • 阳性对照
    • This antibody gave a positive signal in the following Human whole cell lysates: HeLa, Jurkat, HEK293. This antibody gave a positive signal in the following Mouse whole cell lysate: NIH 3T3 (data not shown).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab28658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 48.4 kDa).
IHC-P 1/35. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
EMSA Use at an assay dependent concentration. PubMed: 20691152
IP Use at an assay dependent concentration.

靶标

  • 功能Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking a RNA moiety.
  • 序列相似性Belongs to the Nudix hydrolase family. DCP2 subfamily.
    Contains 1 nudix hydrolase domain.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm > P-body. Nucleus. Predominantly cytoplasmic, in processing bodies (PB). A minor amount is nuclear.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DCP2 antibody
    • DCP2 decapping enzyme homolog (S. cerevisiae) antibody
    • DCP2, S. cerevisiae, homolog of antibody
    • DCP2_HUMAN antibody
    • decapping enzyme 2, S. cerevisiae, homolog of antibody
    • decapping enzyme homolog (S. cerevisiae) antibody
    • FLJ33245 antibody
    • hDpc antibody
    • m7GpppN-mRNA hydrolase antibody
    • mRNA decapping enzyme 2 antibody
    • mRNA-decapping enzyme 2 antibody
    • Nucleoside diphosphate-linked moiety X motif 20 antibody
    • nudix (nucleoside diphosphate linked moiety X)-type motif 20 antibody
    • Nudix motif 20 antibody
    • NUDT20 antibody
    see all

Anti-Dcp2 antibody 图像

  • ICC/IF image of ab28658 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab28658, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • Image courtesy of Human Protein Atlas
    ab28658 stainning Dcp2 in paraffin embedded human kidney. Kidney tissue sections (4 µm) were incubated with ab28658 (1/35 dilution) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
    ab28658 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
  • All lanes : Anti-Dcp2 antibody (ab28658) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human Dcp2 peptide (ab30458) at 1 µg/ml
    Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human Dcp2 peptide (ab30458) at 1 µg/ml
    Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Human Dcp2 peptide (ab30458) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 48.4 kDa
    Observed band size : 52,58 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes
  • Dcp2 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to Dcp2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab28658.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 52,58kDa: Dcp2.

Anti-Dcp2 antibody (ab28658)参考文献

This product has been referenced in:
  • Chiang PY  et al. Phosphorylation of mRNA decapping protein Dcp1a by the ERK signaling pathway during early differentiation of 3T3-L1 preadipocytes. PLoS One 8:e61697 (2013). WB ; Human . Read more (PubMed: 23637887) »
  • Kim CW  et al. Tristetraprolin controls the stability of cIAP2 mRNA through binding to the 3'UTR of cIAP2 mRNA. Biochem Biophys Res Commun 400:46-52 (2010). EMSA ; Human . Read more (PubMed: 20691152) »

See all 2 Publications for this product

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