概述

  • 产品名称
  • 描述
    兔多克隆抗体to Dcp1a
  • 经测试应用
    适用于: WB, ICC/IF, IHC-P, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Dcp1a.

    (Peptide available as ab71605.)

  • 阳性对照
    • This antibody gave a positive signal in the following Human Whole cell Lysates: HeLa, Jurkat and HepG2 This antibody gave a positive result in IHC in the following FFPE tissue: Human placenta

性能

应用

Our Abpromise guarantee covers the use of ab47811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 63 kDa).

Abcam recommends using milk as the blocking agent.

ICC/IF 1/800.
IHC-P Use a concentration of 5 µg/ml.
IP Use at an assay dependent concentration.

靶标

  • 功能
    Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.
  • 组织特异性
    Detected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas.
  • 序列相似性
    Belongs to the DCP1 family.
  • 细胞定位
    Cytoplasm > P-body. Nucleus. Co-localizes with NANOS3 in the processing bodies (By similarity). Predominantly cytoplasmic, in processing bodies (PB). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DCP1 decapping enzyme homolog A antibody
    • Dcp1a antibody
    • DCP1A_HUMAN antibody
    • Decapping enzyme hDcp1a antibody
    • Decapping mRNA 1A antibody
    • HSA275986 antibody
    • mRNA decapping enzyme 1A antibody
    • mRNA-decapping enzyme 1A antibody
    • Nbla00360 antibody
    • Putative protein product of Nbla00360 antibody
    • Smad4 interacting transcriptional co activator antibody
    • Smad4-interacting transcriptional co-activator antibody
    • Smad4-interacting transcriptional co-activator antibody
    • SMAD4IP1 antibody
    • SMIF antibody
    • Transcription factor SMIF antibody
    see all

图片

  • IHC image of Dcp1a staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47811, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Dcp1a antibody (ab47811) at 1 mg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) WCL at 10 µg
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) WCL
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) WCL

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 63 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 120 kDa (possible non-specific binding),90 kDa (possible non-specific binding).

    Exposure time : 12 minutes

    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab47811 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

  • Dcp1a was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to Dcp1a and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab47811.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 75kDa: Dcp1a.
  • ab47811 (1/800) stainning Dcp1a in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

    See Abreview

文献

This product has been referenced in:
  • Da Ros M  et al. FYCO1 and autophagy control the integrity of the haploid male germ cell-specific RNP granules. Autophagy 13:302-321 (2017). Read more (PubMed: 27929729) »
  • Hopkins KC  et al. Virus-induced translational arrest through 4EBP1/2-dependent decay of 5'-TOP mRNAs restricts viral infection. Proc Natl Acad Sci U S A 112:E2920-9 (2015). Read more (PubMed: 26038567) »

See all 12 Publications for this product

客户评价及客户问答

Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Human Cell lysate - whole cell (Epethelial cells)
Specification
Epethelial cells
Total protein in input
300 µg
Username

Abcam user community

Verified customer

提交于 Aug 26 2014

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (epethelial cells)
Specification
epethelial cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Aug 25 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Sample
Human Cell (epethelial cells)
Specification
epethelial cells
Permeabilization
Yes - Triton X100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 25 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Sample
Rhesus monkey Cell (Rhesus monkey kidney cells)
Specification
Rhesus monkey kidney cells
Permeabilization
Yes - 0.2% Triton X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TritonX100 in Phosphate Buffered Saline
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Username

Dr. Harendra Chahar

Verified customer

提交于 Sep 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TritonX100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Dr. Harendra Chahar

Verified customer

提交于 Sep 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk Mcmanus

Verified customer

提交于 May 28 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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