Anti-DCLK3抗体(ab106653)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to DCLK3
  • 特异性
    ab106653 is predicted not to cross-react with other DCLK proteins.
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A 14 amino acid synthetic peptide near the N terminus of Human DCLK3 (NP_208382).

  • 阳性对照
    • K562 cell lysate; Human brain tissue.

性能

应用

Our Abpromise guarantee covers the use of ab106653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 74 kDa.

靶标

  • 序列相似性
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • 细胞定位
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BC056929 antibody
    • C730036H08 antibody
    • CLICK I and II related; CLr antibody
    • Click I II related antibody
    • CLR antibody
    • Dcamkl3 antibody
    • DCDC3C antibody
    • DCK3 antibody
    • Dclk3 antibody
    • DCLK3_HUMAN antibody
    • Doublecortin and CaM kinase like 3 antibody
    • Doublecortin domain-containing protein 3C antibody
    • Doublecortin-like and CAM kinase-like 3 antibody
    • Doublecortin-like kinase 3 antibody
    • hCG_16695 antibody
    • KIAA1765 antibody
    • RGD1309232 antibody
    • Serine/threonine-protein kinase DCLK3 antibody
    see all

图片

  • Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse brain tissue labeling DCLK3 with Anti-DCLK3 antibody (ab106653) at 5μg/ml.
  • Lane 1 : Anti-DCLK3 antibody (ab106653) at 1 µg/ml
    Lane 2 : Anti-DCLK3 antibody (ab106653) at 2 µg/ml

    Lane 1 : K562 cell lysate
    Lane 2 : K562 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 74 kDa
  • ab106653, at 20µg/ml, staining DCLK3 in Human brain tissue by Immunohistochemistry.

文献

ab106653 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Loading amount
65 µg
Gel Running Conditions
Reduced Denaturing (Novex 4-12% Bis-Tris midi-gel)
Sample
Mouse Tissue lysate - whole (liver)
Specification
liver
Treatment
L1: control, L2: 500ug/kg TCDD for 6hrs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Ms. Stephenie Prokopec

Verified customer

提交于 Feb 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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