Anti-DCK抗体[OTI16E12] (ab118994)
Key features and details
- Mouse monoclonal [OTI16E12] to DCK
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
- Isotype: IgG1
概述
-
产品名称
Anti-DCK抗体[OTI16E12]
参阅全部 DCK 一抗 -
描述
小鼠单克隆抗体[OTI16E12] to DCK -
宿主
Mouse -
经测试应用
适用于: Flow Cyt (Intra), WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human DCK aa 1-300. (NP_000779) produced in HEK293T cells.
Database link: P27707 -
阳性对照
- WB: HEK293T cells transfected with pCMV6-ENTRY DCK. Flow Cyt (Intra): HEK-293T transfected with DCK, HeLa and Jurkat cells.
-
常规说明
The clone number has been updated from 16E12 to OTI16E12, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
-
纯度
Affinity purified -
纯化说明
Purified from cell culture supernatant by affinity chromatography -
克隆
单克隆 -
克隆编号
OTI16E12 -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab118994于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
WB |
1/2000. Predicted molecular weight: 31 kDa.
|
说明 |
---|
Flow Cyt (Intra)
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
1/2000. Predicted molecular weight: 31 kDa. |
靶标
-
功能
Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents. -
序列相似性
Belongs to the DCK/DGK family. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 1633 Human
- Omim: 125450 Human
- SwissProt: P27707 Human
- Unigene: 709 Human
-
别名
- dCK antibody
- DCK protein antibody
- DCK_HUMAN antibody
see all
图片
-
All lanes : Anti-DCK antibody [OTI16E12] (ab118994) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY DCK cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 31 kDa -
Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either DCK overexpress plasmid (Red) or empty vector control plasmid (Blue) were stained for DCK using ab118994 at 1/100 dilution.
-
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for DCK using ab118994 (Red) compared to a nonspecific negative control antibody (Blue).
-
Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells using ab118994 at 1/100 (Red), compared to a nonspecific negative control antibody (Blue).
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab118994 尚未被引用在任何文献中。