概述

  • 产品名称Anti-DCK抗体
    参阅全部 DCK 一抗
  • 描述
    兔多克隆抗体to DCK
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Recombinant fragment corresponding to a region within amino acids 1 - 216 of Human DCK.

  • 阳性对照
    • 293T, A431, H1299, HeLa, HepG2, MOLT4 or Raji cell lines.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab96599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 31 kDa.
ICC/IF 1/100 - 1/200.

靶标

  • 功能Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.
  • 序列相似性Belongs to the DCK/DGK family.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • dCK antibody
    • DCK protein antibody
    • DCK_HUMAN antibody
    • Deoxycytidine kinase antibody
    • Deoxynucleoside kinase antibody
    • EC 2.7.1 antibody
    • EC 2.7.1.74 antibody
    • MGC117410 antibody
    • MGC138632 antibody
    • OTTHUMP00000219118 antibody
    • OTTHUMP00000219119 antibody
    see all

Anti-DCK antibody 图像

  • Anti-DCK antibody (ab96599) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 31 kDa
  • ab96599, at 1/200 dilution, staining DCK in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.

Anti-DCK antibody (ab96599)参考文献

This product has been referenced in:
  • Kerr M  et al. Deoxycytidine kinase expression underpins response to gemcitabine in bladder cancer. Clin Cancer Res 20:5435-45 (2014). WB . Read more (PubMed: 25224279) »

See 1 Publication for this product

Product Wall

rdetails and for your cooperation.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused.
I appreciate the time you have spent on these experiments in the laboratory and it is disappointi...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (murine pancreatic adenocarcinoma)
Specification murine pancreatic adenocarcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Kris Frese

Verified customer

提交于 Jan 23 2012

Application Western blot
Sample Mouse Tissue lysate - whole (multiple tissues)
Loading amount 40 µg
Specification multiple tissues
Gel Running Conditions Reduced Denaturing (4-12% gradient)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Kris Frese

Verified customer

提交于 Jan 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"