概述

  • 产品名称Anti-DCK抗体
    参阅全部 DCK 一抗
  • 描述
    兔多克隆抗体to DCK
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (ATPETCLHRI YLRGRNEEQG IPLEYLEKLH YKHESWLLHR TLKTNFDYLQ) of human DCK (NP_000779).

  • 阳性对照
    • Fetal heart lysate

性能

应用

Our Abpromise guarantee covers the use of ab83046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:62500.

靶标

Anti-DCK antibody 图像

  • Anti-DCK antibody (ab83046) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 31 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

Anti-DCK antibody (ab83046)参考文献

This product has been referenced in:
  • Bieghs L  et al. The effects of forodesine in murine and human multiple myeloma cells. Adv Hematol 2010:131895 (2010). WB ; Mouse . Read more (PubMed: 20981156) »

See 1 Publication for this product

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"