Anti-DCK抗体(ab151966)
Key features and details
- Rabbit polyclonal to DCK
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DCK抗体
参阅全部 DCK 一抗 -
描述
兔多克隆抗体to DCK -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 1-260 of Human DCK (Uniprot ID#P27707)
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阳性对照
- 293T, A431, HeLa or HepG2 whole cell lysate (ab7900), Saos2 xenograft tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab151966于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/5000 - 1/20000. Predicted molecular weight: 30 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/5000 - 1/20000. Predicted molecular weight: 30 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents. -
序列相似性
Belongs to the DCK/DGK family. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 1633 Human
- Omim: 125450 Human
- SwissProt: P27707 Human
- Unigene: 709 Human
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别名
- dCK antibody
- DCK protein antibody
- DCK_HUMAN antibody
see all
图片
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All lanes : Anti-DCK antibody (ab151966) at 1/10000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 30 kDa
12% SDS PAGE -
Immunohistochemical analysis of paraffin embedded Human Saos2 xenograft tissue labeling cytosolic DCK with ab151966 at a 1/500 dilution.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab151966 被引用在 9 文献中.
- Wu B et al. Deoxycytidine Kinase (DCK) Mutations in Human Acute Myeloid Leukemia Resistant to Cytarabine. Acta Haematol 144:534-541 (2021). PubMed: 33626530
- Zhao Y et al. Gemcitabine resistance in triple-negative breast cancer cells can be reverted by Drosophila melanogaster deoxyribonucleoside kinase in the nucleus or cytosol. Oncol Lett 20:247 (2020). PubMed: 32973960
- Dahn ML et al. Decitabine Response in Breast Cancer Requires Efficient Drug Processing and Is Not Limited by Multidrug Resistance. Mol Cancer Ther 19:1110-1122 (2020). PubMed: 32156786
- Zhang Y et al. Role of Damage DNA-Binding Protein 1 in Pancreatic Cancer Progression and Chemoresistance. Cancers (Basel) 11:N/A (2019). PubMed: 31842285
- Patel GK et al. Exosomes confer chemoresistance to pancreatic cancer cells by promoting ROS detoxification and miR-155-mediated suppression of key gemcitabine-metabolising enzyme, DCK. Br J Cancer 116:609-619 (2017). PubMed: 28152544
- Cruickshank MN et al. Systematic chemical and molecular profiling of MLL-rearranged infant acute lymphoblastic leukemia reveals efficacy of romidepsin. Leukemia 31:40-50 (2017). PubMed: 27443263
- Shan J et al. The C/ebp-Atf response element (CARE) location reveals two distinct Atf4-dependent, elongation-mediated mechanisms for transcriptional induction of aminoacyl-tRNA synthetase genes in response to amino acid limitation. Nucleic Acids Res 44:9719-9732 (2016). WB ; Human . PubMed: 27471030
- Huang YH et al. Insights from HuR biology point to potential improvement for second-line ovarian cancer therapy. Oncotarget 7:21812-24 (2016). PubMed: 26943573
- Wang C et al. Establishment of human pancreatic cancer gemcitabine-resistant cell line with ribonucleotide reductase overexpression. Oncol Rep 33:383-90 (2015). PubMed: 25394408