Anti-DCK抗体[16E12] (ab118994)

概述

  • 产品名称Anti-DCK抗体[16E12]
    参阅全部 DCK 一抗
  • 描述
    小鼠单克隆抗体[16E12] to DCK
  • 经测试应用适用于: WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein corresponding to Human DCK (NP_000779) produced in HEK293T cells.

  • 阳性对照
    • HEK293T cells transfected with pCMV6-ENTRY DCK. HeLa cells. Jurkat cells.
  • 常规说明Dilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

性能

应用

Our Abpromise guarantee covers the use of ab118994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 31 kDa.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.
  • 序列相似性Belongs to the DCK/DGK family.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • dCK antibody
    • DCK protein antibody
    • DCK_HUMAN antibody
    • Deoxycytidine kinase antibody
    • Deoxynucleoside kinase antibody
    • EC 2.7.1 antibody
    • EC 2.7.1.74 antibody
    • MGC117410 antibody
    • MGC138632 antibody
    • OTTHUMP00000219118 antibody
    • OTTHUMP00000219119 antibody
    see all

Anti-DCK antibody [16E12] 图像

  • All lanes : Anti-DCK antibody [16E12] (ab118994) at 1/2000 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY DCK cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 31 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC210767)
  • HEK293T cells transfected with either DCK overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab118994 at 1/100, and then analyzed by flow cytometry.
  • Flow cytometric analysis of HeLa cells, using ab118994 at 1/100 (Red) compared to a nonspecific negative control antibody (Blue).
  • Flow cytometric analysis of Jurkat cells, using ab118994 at 1/100 (Red), compared to a nonspecific negative control antibody (Blue).

Anti-DCK antibody [16E12] (ab118994)参考文献

ab118994 has not yet been referenced specifically in any publications.

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