Anti-DCDC2抗体(ab45868)

概述

  • 产品名称Anti-DCDC2抗体
    参阅全部 DCDC2 一抗
  • 描述
    山羊多克隆抗体to DCDC2
  • 经测试应用适用于: ELISA, WB, IHC-FoFr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Dog, Pig
  • 免疫原

    Synthetic peptide: C-DGEKANKDA EQKED, corresponding to Internal sequence amino acids 352-365 of Human DCDC2

  • 阳性对照
    • COS7 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab45868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IHC-FoFr 1/200.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能May be involved in neuronal migration during development of the cerebral neocortex.
  • 组织特异性Ubiquitously expressed. In brain, highly expressed in the entorhinal cortex, inferior temporal cortex, medial temporal cortex, hypothalamus, amygdala and hippocampus.
  • 疾病相关Defects in DCDC2 may be a cause of susceptibility to dyslexia type 2 (DYX2) [MIM:600202]; also known as specific reading disability type 2. Dyslexia is a relatively common, complex cognitive disorder that affects 5% to 10% of school-aged children. The disorder is characterized by an impairment of reading performance despite adequate motivational, educational and intellectual opportunities and in the absence of sensory or neurological disability.
  • 序列相似性Contains 2 doublecortin domains.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DCDC 2 antibody
    • DCDC2 antibody
    • DCDC2_HUMAN antibody
    • DCDC2A antibody
    • Doublecortin domain containing 2 antibody
    • doublecortin domain containing protein 2 antibody
    • Doublecortin domain-containing protein 2 antibody
    • HGNC:18141 antibody
    • KIAA1154 antibody
    • Protein RU2S antibody
    • RU 2 antibody
    • RU2 antibody
    • RU2S antibody
    • RU2S protein antibody
    see all

Anti-DCDC2 antibody 图像

  • ab45868 (5µg/ml) staining of DCDC2 in paraffin embedded Human Cerebral Cortex following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows cytoplasm staining in select neurons.

  • ab45868 staining rat optic nerve sections by IHC-FoFr. The animal was perfused with 4% paraformaldehyde and further post fixed with 4% paraformaldehyde overnight. The tissues were cryoprotected and sectioned using a cryostat. Staining with ab45868 at a 1/200 dilution in 0.3% TritonX with 0.1x PBS in 10% donkey serum was performed for 1h at 24°C. A donkey anti-goat Alexa488 polyclonal antibody at 1/1000 was used as the secondary antibody. β III tubulin can be observed in red (Alexa fluro 568 secondary). DCDC2 is expressed in the optic nerve.

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  • All lanes : Anti-DCDC2 antibody (ab45868) at 0.2 µg/ml

    Lane 1 : COS7 cell lysate transfected with full length recombinant human DCDC2
    Lane 2 : Untransfected control COS7 cells


    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Primary incubation was 1 hour. Detected by chemiluminescence.
  • All lanes : Anti-DCDC2 antibody (ab45868) at 1/500 dilution

    Lane 1 : adult mouse heart
    Lane 2 : adult mouse brain
    Lane 3 : three-month old mouse brain
    Lane 4 : 5 week old mouse brain
    Lane 5 : mouse hippcampus including dentate gyrus
    Lane 6 : mouse DRG
    Lane 7 : primary mouse neuronal culture
    Lane 8 : mouse astrocyte culture

    Lysates/proteins at 20 µg per lane.

    Secondary
    DAKO cytomation Conjugation: HRP
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 15 kDa (possible non-specific binding),37 kDa (possible non-specific binding).

    Exposure time : 1 minute

    This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

    Lane 1: adult mouse heart was run as a negative control Lanes 6, 7 & 8 were run as positive controls

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Anti-DCDC2 antibody (ab45868)参考文献

ab45868 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (DRG neurons culture)
Specification DRG neurons culture
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Apr 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Retina, eye)
Specification Retina, eye
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Dec 06 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Brain, heart primary cells)
Loading amount 20 µg
Specification Brain, heart primary cells
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Jun 02 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"