概述

  • 产品名称Anti-DCAF13抗体
    参阅全部 DCAF13 一抗
  • 描述
    兔多克隆抗体to DCAF13
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Hamster, Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster
  • 免疫原

    Synthetic peptide: WSPAGRATEM KVKMLSRNPD NYVRETKLDL QRVPRNYDPA LHPFEVPREY , corresponding to a region within N terminal amino acids 144 - 193 of Human DCAF13 (NP_056235)

  • 阳性对照
    • HepG2 cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab83546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-DCAF13 antibody 图像

  • Anti-DCAF13 antibody (ab83546) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 51 kDa
    Observed band size : 67 kDa (why is the actual band size different from the predicted?)

Anti-DCAF13 antibody (ab83546)参考文献

ab83546 has not yet been referenced specifically in any publications.

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