概述

  • 产品名称Anti-DC-SIGN抗体[EPR5588]
    参阅全部 DC-SIGN 一抗
  • 描述
    兔单克隆抗体[EPR5588] to DC-SIGN
  • 经测试应用适用于: WBmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human DC-SIGN aa 1-100.
    Database link: Q9NNX6

  • 阳性对照
    • WB: HACAT, fetal skin, fetal artery and Human small intestine lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab124828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 46 kDa).
  • 应用说明Is unsuitable for IHC-P.
  • 靶标

    • 功能Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, cytomegalovirus gB, HCV E2, dengue virus gE, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA.
      On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells.
    • 组织特异性Predominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes.
    • 序列相似性Contains 1 C-type lectin domain.
    • 结构域The tandem repeat domain, also called neck domain, mediates oligomerization.
    • 细胞定位Secreted and Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • C type lectin domain family 4 member L antibody
      • C-type lectin domain family 4 member L antibody
      • CD 209 antibody
      • CD209 antibody
      • CD209 antigen antibody
      • CD209 antigen-like protein A antibody
      • CD209 molecule antibody
      • CD209_HUMAN antibody
      • Cd209a antibody
      • CDSIGN antibody
      • CIRE antibody
      • CLEC4L antibody
      • DC SIGN antibody
      • DC SIGN1 antibody
      • DC-SIGN antibody
      • DC-SIGN1 antibody
      • DCSIGN antibody
      • Dendritic cell specific ICAM 3 grabbing nonintegrin 1 antibody
      • Dendritic cell specific ICAM3 grabbing nonintegrin 1 antibody
      • Dendritic cell-specific ICAM-3-grabbing non-integrin 1 antibody
      • Dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin antibody
      • Dengue fever, protection against, included antibody
      • Dentritic Cell Specific ICAM3 Grabbing Nonintegrin antibody
      • HIV GP120 Binding Protein antibody
      • MGC129965 antibody
      • MGC130443 antibody
      • SIGN-R1 antibody
      • SIGNR5 antibody
      see all

    Anti-DC-SIGN antibody [EPR5588] 图像

    • Anti-DC-SIGN antibody [EPR5588] (ab124828) at 1/1000 dilution (purified) + Human skin lysate at 20 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 46 kDa

      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-DC-SIGN antibody [EPR5588] (ab124828) at 1/1000 dilution (unpurified)

      Lane 1 : HACAT cell lysate
      Lane 2 : Fetal skin lysate
      Lane 3 : Fetal artery lysate
      Lane 4 : Human small intestine lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size : 46 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-DC-SIGN antibody [EPR5588] (ab124828)参考文献

    This product has been referenced in:
    • Linley A  et al. Lectin binding to surface Ig variable regions provides a universal persistent activating signal for follicular lymphoma cells. Blood 126:1902-10 (2015). Read more (PubMed: 26194765) »

    See 1 Publication for this product

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