概述

  • 产品名称Anti-DC-SIGN抗体
    参阅全部 DC-SIGN 一抗
  • 描述
    兔多克隆抗体to DC-SIGN
  • 经测试应用适用于: ICC/IF, IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human DC-SIGN aa 384-404.
    (Peptide available as ab6091)

  • 阳性对照
    • Human placenta tissue lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab5715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 44 kDa.Can be blocked with Human DC-SIGN peptide (ab6091).

靶标

  • 功能Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, cytomegalovirus gB, HCV E2, dengue virus gE, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA.
    On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells.
  • 组织特异性Predominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes.
  • 序列相似性Contains 1 C-type lectin domain.
  • 结构域The tandem repeat domain, also called neck domain, mediates oligomerization.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C type lectin domain family 4 member L antibody
    • C-type lectin domain family 4 member L antibody
    • CD 209 antibody
    • CD209 antibody
    • CD209 antigen antibody
    • CD209 antigen-like protein A antibody
    • CD209 molecule antibody
    • CD209_HUMAN antibody
    • Cd209a antibody
    • CDSIGN antibody
    • CIRE antibody
    • CLEC4L antibody
    • DC SIGN antibody
    • DC SIGN1 antibody
    • DC-SIGN antibody
    • DC-SIGN1 antibody
    • DCSIGN antibody
    • Dendritic cell specific ICAM 3 grabbing nonintegrin 1 antibody
    • Dendritic cell specific ICAM3 grabbing nonintegrin 1 antibody
    • Dendritic cell-specific ICAM-3-grabbing non-integrin 1 antibody
    • Dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin antibody
    • Dengue fever, protection against, included antibody
    • Dentritic Cell Specific ICAM3 Grabbing Nonintegrin antibody
    • HIV GP120 Binding Protein antibody
    • MGC129965 antibody
    • MGC130443 antibody
    • SIGN-R1 antibody
    • SIGNR5 antibody
    see all

Anti-DC-SIGN antibody 图像

  • ab5715 at 0.5 µg /ml staining approximately 44kDa of human CD209 in Human placenta lysate by Western blot (ECL).
  • Immunohistochemistry of DC-SIGN in human lymph node tissue with DC-SIGN antibody at 10 µg/ml.
  • Lane 1 : Anti-DC-SIGN antibody (ab5715) at 1 µg/ml
    Lane 2 : Anti-DC-SIGN antibody (ab5715) at 2 µg/ml

    Lane 1 : Human placenta
    Lane 2 : Human placenta


    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of DC-SIGN in Human Lymph Node cells using ab5715 at 20 ug/ml.

Anti-DC-SIGN antibody (ab5715)参考文献

This product has been referenced in:
  • Moutsopoulos NM  et al. Defective neutrophil recruitment in leukocyte adhesion deficiency type I disease causes local IL-17-driven inflammatory bone loss. Sci Transl Med 6:229ra40 (2014). Read more (PubMed: 24670684) »

See 1 Publication for this product

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