概述

  • 产品名称Anti-DBR1抗体
    参阅全部 DBR1 一抗
  • 描述
    小鼠多克隆抗体to DBR1
  • 特异性This antibody is reactive against mammalian transfected lysate.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    DBR1 (NP_057300.2, 1 a.a. ~ 544 a.a) full-length human protein.

  • 阳性对照
    • DBR1 transfected 293T cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab67891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 62 kDa).

靶标

  • 功能Cleaves the 2'-5' phosphodiester linkage at the branch point of lariat intron pre-mRNAs after splicing and converts them into linear molecules that are subsequently degraded. It thereby facilitates ribonucleotide turnover. It may also participate in retrovirus replication via a RNA lariat intermediate in cDNA synthesis.
  • 序列相似性Belongs to the lariat debranching enzyme family.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DBR1 antibody
    • DBR1_HUMAN antibody
    • Debranching enzyme homolog 1 (S. cerevisiae) antibody
    • Lariat debranching enzyme antibody
    • RNA lariat debranching enzyme antibody
    see all

Anti-DBR1 antibody 图像

  • All lanes : Anti-DBR1 antibody (ab67891) at 1/500 dilution

    Lane 1 : DBR1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 62 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of purified antibody to DBR1 (ab67891) on HeLa cells. Antibody concentration was 10 ug/ml.

Anti-DBR1 antibody (ab67891)参考文献

ab67891 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 10 cells
Gel Running Conditions Reduced Denaturing (12.5%)
Sample Xenopus laevis Cell lysate - nuclear (oocyte)
Specification oocyte
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Feb 26 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"