Anti-DBF4抗体[EPR5635] (ab124707)


  • 产品名称Anti-DBF4抗体[EPR5635]
    参阅全部 DBF4 一抗
  • 描述
    兔单克隆抗体[EPR5635] to DBF4
  • 经测试应用适用于: WBmore details
    不适用于: Flow Cyt,ICC/IF,IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human DBF4 protein (Q9UBU7).

  • 阳性对照
    • PC-3, Jurkat, SW480, and HepG2 cell lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab124707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Predicted molecular weight: 77 kDa.
  • 应用说明Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • 靶标

    • 功能Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
    • 组织特异性Highly expressed in testis and thymus. Expressed also in most cancer cells lines.
    • 序列相似性Contains 2 BRCT domains.
      Contains 1 DBF4-type zinc finger.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Activator of S phase kinase antibody
      • ASK antibody
      • CHIF antibody
      • Chiffon homolog A antibody
      • DBF4 antibody
      • DBF4 homolog (S. cerevisiae) antibody
      • DBF4 homolog antibody
      • DBF4 type zinc finger containing protein 1 antibody
      • DBF4 zinc finger A antibody
      • DBF4 zinc finger antibody
      • DBF4, S. cerevisiae, homolog of antibody
      • DBF4-type zinc finger-containing protein 1 antibody
      • DBF4A antibody
      • DBF4A_HUMAN antibody
      • Dumbbell former 4, S. cerevisiae, homolog of antibody
      • OTTHUMP00000161661 antibody
      • Protein DBF4 homolog A antibody
      • ZDBF1 antibody
      • Zinc finger, DBF-type containing 1 antibody
      see all

    Anti-DBF4 antibody [EPR5635] 图像

    • All lanes : Anti-DBF4 antibody [EPR5635] (ab124707) at 1/10000 dilution

      Lane 1 : PC-3 cell lysates
      Lane 2 : Jurkat cell lysates
      Lane 3 : SW480 cell lysates
      Lane 4 : HepG2 cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 77 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-DBF4 antibody [EPR5635] (ab124707)参考文献

    ab124707 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (hEK293)
    Total protein in input 300 µg
    Immuno-precipitation step Other - protein G dynabeads
    Specification hEK293

    Dr. Edyta Marcon

    Verified customer

    提交于 Jun 27 2015

    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing (4-12 Bis tris)
    Sample Human Cell lysate - whole cell (293T)
    Specification 293T
    Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

    Abcam user community

    Verified customer

    提交于 Aug 14 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HEK293T)
    Loading amount 50 µg
    Specification HEK293T
    Gel Running Conditions Reduced Denaturing (8% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

    Abcam user community

    Verified customer

    提交于 Feb 27 2012