• 产品名称
  • 描述
    兔多克隆抗体to DBF4
  • 特异性
    ab70134 detects endogenous levels of total DBF4 protein.
  • 经测试应用
    适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide derived from the N terminus of human DBF4.

  • 阳性对照
    • NIH3T3 cell extracts treated with H2O2 (100µM, 30mins), COS7 cell extracts treated with PMA (125mg/ml, 30mins), and NIH3T3 cell extracts treated with both H2O2 (100µM, 30mins) and PMA (125mg/ml, 30mins).


  • 形式
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab70124 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • 克隆
  • 同种型
  • 研究领域


Our Abpromise guarantee covers the use of ab70134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
ELISA 1/10000.


  • 功能
    Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
  • 组织特异性
    Highly expressed in testis and thymus. Expressed also in most cancer cells lines.
  • 序列相似性
    Contains 2 BRCT domains.
    Contains 1 DBF4-type zinc finger.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activator of S phase kinase antibody
    • ASK antibody
    • CHIF antibody
    • Chiffon homolog A antibody
    • DBF4 antibody
    • DBF4 homolog (S. cerevisiae) antibody
    • DBF4 homolog antibody
    • DBF4 type zinc finger containing protein 1 antibody
    • DBF4 zinc finger A antibody
    • DBF4 zinc finger antibody
    • DBF4, S. cerevisiae, homolog of antibody
    • DBF4-type zinc finger-containing protein 1 antibody
    • DBF4A antibody
    • DBF4A_HUMAN antibody
    • Dumbbell former 4, S. cerevisiae, homolog of antibody
    • OTTHUMP00000161661 antibody
    • Protein DBF4 homolog A antibody
    • ZDBF1 antibody
    • Zinc finger, DBF-type containing 1 antibody
    see all

Anti-DBF4 antibody 图像

  • All lanes : Anti-DBF4 antibody (ab70134) at 1/500 dilution

    Lane 1 : NIH3T3 cell extracts treated with H2O2 (100µM, 30mins) at 5 µg
    Lane 2 : COS7 cell extracts treated with PMA (125mg/ml, 30mins)
    Lane 3 : NIH3T3 cell extracts treated with H2O2 (100µM, 30mins) and PMA (125mg/ml, 30mins) with immunising peptide at 5 µg

    Predicted band size : 77 kDa
    Observed band size : 77 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab70134 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70134, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-DBF4 antibody (ab70134)参考文献

ab70134 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us in regards to Anti-DBF4 antibody (ab70134). I have been assured by the source of this antibody that the Western blot performed and displayed on the datasheet was performed with endogenous expression of DBF4 from NIH3T3 a...

Read More