概述

  • 产品名称Anti-DAZAP1抗体
    参阅全部 DAZAP1 一抗
  • 描述
    兔多克隆抗体to DAZAP1
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A region within synthetic peptide: QAAPDMSKPP TAQPDFPYGQ YGLGSYSPAP PGCGPHFVYS LMVRLSSDVA, corresponding to amino acids 240-289 of Human DAZAP1

  • 阳性对照
    • HepG2 cell lysate, Human kidney.

性能

应用

Our Abpromise guarantee covers the use of ab51010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA 1/312500.

Titre using peptide based assay 1/312500.

靶标

  • 功能RNA-binding protein, which may be required during spermatogenesis.
  • 组织特异性Mainly expressed in testis. Expressed to a lower level in thymus. Weakly or not expressed in heart, liver, brain, placenta, lung, skeletal muscle, kidney and pancreas.
  • 序列相似性Contains 2 RRM (RNA recognition motif) domains.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear at some stages of spermatozoides development. In midpachytene spermatocytes, it is localized in both the cytoplasm and the nuclei and is clearly excluded from the sex vesicles. In round spermatids, it localizes mainly in the nuclei, whereas in elongated spermatids, it localizes to the cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DAZ associated protein 1 antibody
    • DAZ-associated protein 1 antibody
    • Dazap1 antibody
    • DAZP1_HUMAN antibody
    • Deleted in azoospermia associated protein 1 antibody
    • Deleted in azoospermia-associated protein 1 antibody
    see all

Anti-DAZAP1 antibody 图像

  • Lane 1 : Marker
    Lane 2 : Anti-DAZAP1 antibody (ab51010) at 0.5 µg/ml

    Lane 1 : As above
    Lane 2 : HepG2 cell lysate at 10 µg with skim milk/ PBS at 5 %

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 43 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • Ab51010 at 4.0 µg/ml staining epithelial cells of renal tubule from human kidney; paraffin embedded; magnification 400x.

Anti-DAZAP1 antibody (ab51010)参考文献

This product has been referenced in:
  • Hayakawa H  et al. Human proteins that specifically bind to 8-oxoguanine-containing RNA and their responses to oxidative stress. Biochem Biophys Res Commun 403:220-4 (2010). WB ; Human . Read more (PubMed: 21073862) »

See 1 Publication for this product

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