概述

  • 产品名称Anti-DAZAP1抗体
    参阅全部 DAZAP1 一抗
  • 描述
    兔多克隆抗体to DAZAP1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Mouse DAZAP1.

  • 阳性对照
    • This antibody gave a positive signal in Human, Mouse and Rat Testis tissue lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab106851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).

靶标

  • 功能RNA-binding protein, which may be required during spermatogenesis.
  • 组织特异性Mainly expressed in testis. Expressed to a lower level in thymus. Weakly or not expressed in heart, liver, brain, placenta, lung, skeletal muscle, kidney and pancreas.
  • 序列相似性Contains 2 RRM (RNA recognition motif) domains.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear at some stages of spermatozoides development. In midpachytene spermatocytes, it is localized in both the cytoplasm and the nuclei and is clearly excluded from the sex vesicles. In round spermatids, it localizes mainly in the nuclei, whereas in elongated spermatids, it localizes to the cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DAZ associated protein 1 antibody
    • DAZ-associated protein 1 antibody
    • Dazap1 antibody
    • DAZP1_HUMAN antibody
    • Deleted in azoospermia associated protein 1 antibody
    • Deleted in azoospermia-associated protein 1 antibody
    see all

Anti-DAZAP1 antibody 图像

  • All lanes : Anti-DAZAP1 antibody (ab106851) at 1 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : Testis (Mouse) Tissue Lysate
    Lane 3 : Testis (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 46 kDa,57 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

Anti-DAZAP1 antibody (ab106851)参考文献

ab106851 has not yet been referenced specifically in any publications.

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