概述

  • 产品名称Anti-Daxx抗体
    参阅全部 Daxx 一抗
  • 描述
    兔多克隆抗体to Daxx
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human, Monkey
  • 免疫原

    Synthetic peptide corresponding to Human Daxx aa 722-740.
    Sequence:

    TSVATQCDPEEIIVLSDSD


    (Peptide available as ab7879)

  • 阳性对照
    • HeLa whole cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab2017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ICC/IF
  • 应用说明ICC/IF: Use at a concentration of 10 µg/ml.
    Western blot (1:500 to 1:1000) gives a 120 kD band.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Transcription corepressor known to repress transcriptional potential of several sumoylated transcription factors. Down-regulates basal and activated transcription. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. Inhibits transcriptional activation of PAX3 and ETS1 through direct protein-protein interactions. Modulates PAX5 activity; the function seems to involve CREBBP. Acts as an adapter protein in a MDM2-DAXX-USP7 complex by regulating the RING-finger E3 ligase MDM2 ubiquitination activity. Under non-stress condition, in association with the deubiquitinating USP7, prevents MDM2 self-ubiquitination and enhances the intrinsic E3 ligase activity of MDM2 towards TP53, thereby promoting TP53 ubiquitination and subsequent proteasomal degradation. Upon DNA damage, its association with MDM2 and USP7 is disrupted, resulting in increased MDM2 autoubiquitination and consequently, MDM2 degradation, which leads to TP53 stabilization. Acts as histone chaperone that facilitates deposition of histone H3.3. Acts as targeting component of the chromatin remodeling complex ATRX:DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. Does not affect the ATPase activity of ATRX but alleviates its transcription repression activity. Upon neuronal activation associates with regulatory elements of selected immediate early genes where it promotes deposition of histone H3.3 which may be linked to transcriptional induction of these genes. Required for the recruitment of histone H3.3:H4 dimers to PML-nuclear bodies (PML-NBs); the process is independent of ATRX and facilitated by ASF1A; PML-NBs are suggested to function as regulatory sites for the incorporation of newly synthesized histone H3.3 into chromatin. In case of overexpression of centromeric histone variant CENPA (as found in various tumors) is involved in its mislocalization to chromosomes; the ectopic localization involves a heterotypic tetramer containing CENPA, and histones H3.3 and H4 and decreases binding of CTCF to chromatin. Proposed to mediate activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast, in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. Shows restriction activity towards human cytomegalovirus (HCMV).
    • 组织特异性Ubiquitous.
    • 序列相似性Belongs to the DAXX family.
    • 结构域The Sumo interaction motif mediates Sumo binding, and is required both for sumoylation and binding to sumoylated targets.
    • 翻译后修饰Sumoylated with SUMO1 on multiple lysine residues.
      Phosphorylated by HIPK1 upon glucose deprivation.
      Polyubiquitinated; which is promoted by CUL3 and SPOP and results in proteasomal degradation. Ubiquitinated by MDM2; inducing its degradation. Deubiquitinated by USP7; leading to stabilize it.
    • 细胞定位Nucleus. Diffuse nuclear distribution pattern and no comparable dot-like accumulation of isoform 1 and Cytoplasm. Nucleus, nucleoplasm. Nucleus, PML body. Nucleus, nucleolus. Chromosome, centromere. Dispersed throughout the nucleoplasm, in PML/POD/ND10 nuclear bodies, and in nucleoli (Probable). Colocalizes with histone H3.3, ATRX, HIRA and ASF1A at PML-nuclear bodies (PubMed:12953102, PubMed:14990586, PubMed:23222847, PubMed:24200965). Colocalizes with a subset of interphase centromeres, but is absent from mitotic centromeres (PubMed:9645950). Detected in cytoplasmic punctate structures (PubMed:11842083). Translocates from the nucleus to the cytoplasm upon glucose deprivation or oxidative stress (PubMed:12968034). Colocalizes with RASSF1 in the nucleus (PubMed:18566590). Colocalizes with USP7 in nucleoplasma with accumulation in speckled structures (PubMed:16845383).
    • Information by UniProt
    • 数据库链接
    • 别名
      • antibody
      • BING 2 antibody
      • BING2 antibody
      • CENP-C binding protein antibody
      • DAP 6 antibody
      • DAP6 antibody
      • Daxx antibody
      • DAXX_HUMAN antibody
      • Death associated protein 6 antibody
      • Death domain associated protein 6 antibody
      • Death domain associated protein antibody
      • Death domain-associated protein 6 antibody
      • EAP 1 antibody
      • EAP1 antibody
      • ETS1 associated protein 1 antibody
      • ETS1-associated protein 1 antibody
      • Fas binding protein antibody
      • Fas death domain associated protein antibody
      • Fas death domain-associated protein antibody
      • hDaxx antibody
      • MGC126245 antibody
      • MGC126246 antibody
      see all

    Anti-Daxx antibody 图像

    • ab2017 at 10µg/ml staining DAXX in Hela cells by ICC/IF

    Anti-Daxx antibody (ab2017)参考文献

    This product has been referenced in:
    • Dionne KR  et al. Daxx upregulation within the cytoplasm of reovirus-infected cells is mediated by interferon and contributes to apoptosis. J Virol 87:3447-60 (2013). Read more (PubMed: 23302889) »

    See 1 Publication for this product

    Product Wall

    The sensitivity of Western blot is 1-10 ng of protein per band. We detected other antibodies but not this one. It detects the denatured protein, but we have not tested the native protein. It is possible to cross-recognize other proteins.

    This antibody has not been tested for use in immunocytochemistry. It has only been confirmed for Western Blot.

    It has not been tested for immunoprecipitation.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"