Daudi全细胞裂解液(ab3951)

概述

  • 产品名称Daudi全细胞裂解液
  • 常规说明Cell line: Daudi (Human Burkitt's lymphoma).
    Growth media: RPMI 1640 medium with 2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate, and 10% fetal bovine serum.


    Daudi cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • 经测试应用适用于: WBmore details

性能

  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Constituent: 5% Beta mercaptoethanol
  • Concentration information loading...
  • 纯度Whole Cell Lysate
  • 裂解物说明Daudi cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • 研究领域
  • 背景Daudi cells are lymphoblast-like in morphology and contain the Epstein-Barr virus. They are positive for the Fc receptor and certain tumor markers and are used for studies of differentiation and tumorigenicity and for antitumour testing. The lysate is a positive control for: IKKalpha (numerous ab in our catalogue for IKK alpha), IKKbeta (=IKK2) (numerous ab in our catalogue for IKK beta), DR4 (numerous ab in our catalogue for DR4), NFkB p65 (numerous ab in our catalogue for NFkB p65).

应用

Our Abpromise guarantee covers the use of ab3951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Daudi cell lysate is ready to load on SDS-PAGE for Western blotting, 10 - 20 µg per lane is recommended for mini gel.

Daudi whole cell lysate (ab3951)参考文献

ab3951 has not yet been referenced specifically in any publications.

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