Anti-DATF1 antibody [3B1] (ab92868) at 5 µg/ml + HepG2 cell lysate at 25 µg
Secondary Goat anti-Mouse IgG HRP conjugated at 1/2500 dilution
Predicted band size : 244 kDa
Flow Cytometry - Anti-DATF1 antibody (ab92868)
Overlay histogram showing HepG2 cells stained with ab92868 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92868, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Alonso-Orgaz S et al. Proteomic characterization of human coronary thrombus in patients with ST-segment elevation acute myocardial infarction. J Proteomics109C:368-381 (2014).
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