Specificity testing was performed with an ELISA test by competition experiments with the following compounds:
Compounds/cross-reactivity ratio (a):
D.Aspartic acid-Glutaraldehyde-BSA: 1
L.Aspartic acid-Glutaraldehyde-BSA: 1/>50,000
D.Glutamic acid-Glutaraldehyde-BSA: 1/>50,000
L.Glutamic acid-Glutaraldehyde-BSA: 1/>50,000
(a): D.Aspartic-BSA concentration/conjugated close structure concentration at half displacement.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/5000.
1/5000 - 1/10000.
D.Aspartic acid is the D-isomer of aspartic acid also known as aspartate which is one of the 20 natural proteinogenic amino acids, the building blocks of proteins. As its name indicates, aspartic acid is the carboxylic acid analog of asparagine. It is non-essential in mammals, and might serve as an excitatory neurotransmitter in the brain. It is also a metabolite in the urea cycle, and participates in gluconeogenesis.