概述
- 产品名称Anti-DARPP32 (phospho T75)抗体
参阅全部 DARPP32 一抗 - 描述兔多克隆抗体to DARPP32 (phospho T75)
- 特异性This antibody detects endogenous levels of DARPP32 only when phosphorylated at threonine 75.
- 经测试应用适用于: ELISA, WB, IHC-Pmore details
- 种属反应性与反应: Human
预测可用于: Mouse, Rat
- 免疫原
Synthetic phosphopeptide derived from human DARPP32 around the phosphorylation site of threonine 75 (A-Y-phosphoT-P-P).
- 阳性对照
- Human brain tissue.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- 存储溶液Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - 纯度Immunogen affinity purified
- 纯化说明The antibody was purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab51114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | 1/40000. | |
| WB | 1/3000. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa). | |
| IHC-P | Use at an assay dependent concentration. |
靶标
- 功能Inhibitor of protein-phosphatase 1.
- 序列相似性Belongs to the protein phosphatase inhibitor 1 family.
- 翻译后修饰Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
Phosphorylation of Thr-34 is required for activity. - 细胞定位Cytoplasm.
- Information by UniProt
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数据库链接
- Entrez Gene: 84152 Human
- Entrez Gene: 19049 Mouse
- Entrez Gene: 360616 Rat
- Omim: 604399 Human
- SwissProt: Q9UD71 Human
- SwissProt: Q60829 Mouse
- SwissProt: Q6J4I0 Rat
- Unigene: 286192 Human
see all -
别名
- DARPP 32 antibody
- DARPP-32 antibody
- Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody
see all
Anti-DARPP32 (phospho T75) antibody 图像
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Immunohistochemistry (Paraffin-embedded sections) - DARPP32 (phospho T75) antibody (ab51114)Ab51114 staining human DARPP32 in human brain tissue by immunohistochemistry using paraffin embedded tissue. -
Western blot - DARPP32 (phospho T75) antibody (ab51114)All lanes : Anti-DARPP32 (phospho T75) antibody (ab51114) at 1 µg/ml
Lane 1 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 32 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Anti-DARPP32 (phospho T75) antibody (ab51114)参考文献
ab51114 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"