RabMAb

Anti-DARPP32抗体[EP720Y] (ab40801)

概述

  • 产品名称Anti-DARPP32抗体[EP720Y]
    参阅全部 DARPP32 一抗
  • 描述
    兔单克隆抗体[EP720Y] to DARPP32
  • 经测试应用适用于: IHC-Fr, Flow Cyt, ICC/IF, WB, IHC-P, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Pig
  • 免疫原

    corresponding to DARPP32 (N terminal).
    (Peptide available as ab189245)

  • 阳性对照
    • Rat brain, cerebral cortex and hippocampus tissue; Mouse brain and cerebral cortex tissue; Human breast adenocarcinoma; Human fetal brain tissue lysate; Human colon tissue; pig brain tissue.
  • 常规说明

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab40801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/7500.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/50 - 1/100.
WB 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).Can be blocked with DARPP32 peptide (ab189245).

For unpurified use at 1/5000 - 1/50 000. 

IHC-P 1/50.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

IP 1/20.

For unpurified use at 1/50. 

靶标

  • 功能Inhibitor of protein-phosphatase 1.
  • 序列相似性Belongs to the protein phosphatase inhibitor 1 family.
  • 翻译后修饰Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
    Phosphorylation of Thr-34 is required for activity.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DARPP 32 antibody
    • DARPP-32 antibody
    • Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody
    • Dopamine and cAMP regulated neuronal phosphoprotein antibody
    • Dopamine and cAMP regulated phosphoprotein antibody
    • Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody
    • Dopamine and cAMP regulated phosphoprotein DARPP32 antibody
    • Dopamine- and cAMP-regulated neuronal phosphoprotein antibody
    • FLJ20940 antibody
    • IPPD antibody
    • Neuronal phosphoprotein DARPP 32 antibody
    • PPP1R1B antibody
    • PPR1B_HUMAN antibody
    • Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody
    • Protein phosphatase 1 regulatory inhibitor subunit 1B antibody
    • Protein phosphatase 1 regulatory subunit 1B antibody
    see all

Anti-DARPP32 antibody [EP720Y] 图像

  • All lanes : Anti-DARPP32 antibody [EP720Y] (ab40801) at 1/50000 dilution (purified)

    Lane 1 : Rat Hippocampus tissue lysate
    Lane 2 : Rat brain tissue lysate
    Lane 3 : Mouse cerebral cortex tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
    Blocking/Diluting buffer 5% NFDM/TBST
  • Immunohistochemical analysis of paraffin-embedded human colon sections labelling DARPP32 with purified ab40801 at dilution of 1/50. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Immunocytochemistry/Immunofluorescence analysis of mouse brain tissue lysate labelling DARPP32 with purified ab40801 at 1/100 (3.4 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was performed with Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000, 2 μg/mL) was used as the secondary antibody.

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control and is shown in the inset. 

  • ab40801 at 1/20 immunoprecipitating DARPP32 in rat brain whole cell lysate observed at 32 KDa (lanes 1 and 2).

    Lane 1 (input): Rat brain whole cell lysate 10μg

    Lane 2 (+): ab40801 + Rat brain whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40801 in Rat brain whole cell lysate

    For western blotting, ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

     

     

  • Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex sections labelling DARPP32 with purified ab40801 at dilution of 1/50. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex sections labelling DARPP32 with purified ab40801 at dilution of 1/50. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Anti-DARPP32 antibody [EP720Y] (ab40801) at 1/1000 dilution (purified) + Human fetal brain lysate at 15 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
    Blocking/Diluting buffer 5% NFDM/TBST
  • Anti-DARPP32 antibody [EP720Y] (ab40801) at 1/50000 dilution (unpurified) + Rat brain lysate at 10 µg

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
  • Immunohistochemical analysis of human breast adenocarcinoma sections labelling DARPP32 with unpurified ab40801 at a dilution of 1/50. 

  • ab40801 (unpurified) staining DARPP32 in pig brain tissue sections by IHC-Fr (formaldehyde-fixed Frozen sections). Tissue was fixed with formaldehyde (4%), permeabilized with Triton X-100 and blocked with 0.2% milk for 30 minutes at 24°C. Samples were incubated with primary antibody (1/7500 in TBS + 1% Triton X-100 + 0.2% milk) for 24 hours at 4°C. A Biotin-conjugated Sheep polyclonal to rabbit IgG, dilution 1/400, was used as secondary antibody. Endogenous peroxidase was blocked with 0.3% H2O2 in methanol for 20 minutes.

    See Abreview

  • Immunohistochemical analysis of murine brain tissue, staining DARPP32 (red) with unpurified ab40801. Slices were fixed in paraformaldehyde and blocked with 5% horse serum and 0.25% Triton X-100. Samples were incubated with primary antibody (1/200) for 15 hours.

Anti-DARPP32 antibody [EP720Y] (ab40801)参考文献

This product has been referenced in:
  • Straccia M  et al. Human t-DARPP is induced during striatal development. Neuroscience 333:320-30 (2016). WB, IHC-P ; Human . Read more (PubMed: 27475250) »
  • Guo X  et al. VCP recruitment to mitochondria causes mitophagy impairment and neurodegeneration in models of Huntington's disease. Nat Commun 7:12646 (2016). Read more (PubMed: 27561680) »

See all 18 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human)
Gel Running Conditions Reduced Denaturing
Loading amount 30 µg
Treatment alpha synuclein
Specification human
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Ms. sehee oh

Verified customer

提交于 Jul 13 2016

Application Flow Cytometry
Sample Human Cell (Differentiated hNSCs)
Permeabilization Yes - 0.25% Triton X-100 in DPBS
Gating Strategy Undifferentiated Stem Cells (white)
Specification Differentiated hNSCs
Preparation Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Fixation Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 18 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Human Cell (Differentiated hNSCs)
Specification Differentiated hNSCs
Permeabilization Yes - 0.25% Triton X-100 in DPBS
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 17 2015

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Sheep Tissue sections (Brain Median Eminence)
Specification Brain Median Eminence
Permeabilization Yes - 0.01% Triton
Fixative Paraformaldehyde
Username

Dr. Gregroy Conductier

Verified customer

提交于 Oct 24 2014

Application IHC - Wholemount
Sample Zebra finch Tissue (14um brain section)
Specification 14um brain section
Username

Abcam user community

Verified customer

提交于 May 13 2014

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (12% Tris Glycine)
Sample Zebra finch Tissue lysate - other (Brain cytoplasmic fraction)
Specification Brain cytoplasmic fraction
Blocking step Rotiblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 14 2013

Thank you for contacting us.

While there a many targets which express differentially in distinct layers of the neocortex many of these are expressed in the striatum as well. I found these papers to have a number of good targets which seem ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - other (Human brain tissue homogenate)
Loading amount 5 µg
Specification Human brain tissue homogenate
Treatment Sample boiled in sample buffer for 5 mins prior to loading on gel
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Aug 19 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Pig Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Permeabilization Yes - Triton X-100
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 0.2% · Temperature: 24°C
Username

Mr. Marian Hruska-Plochan

Verified customer

提交于 Feb 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"