概述

  • 产品名称
    Anti-DARPP32抗体
    参阅全部 DARPP32 一抗
  • 描述
    兔多克隆抗体to DARPP32
  • 特异性
    Detects endogenous levels of total DARPP-32 protein.
  • 经测试应用
    适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic non-phosphopeptide derived from human DARPP-32 around the phosphorylation site of threonine 75 (A-Y-TP-P-P)

  • 阳性对照
    • Extracts from 293 cells treated with EGF, human brain tissue.

性能

应用

Our Abpromise guarantee covers the use of ab51139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/40000.

靶标

  • 功能
    Inhibitor of protein-phosphatase 1.
  • 序列相似性
    Belongs to the protein phosphatase inhibitor 1 family.
  • 翻译后修饰
    Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
    Phosphorylation of Thr-34 is required for activity.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DARPP 32 antibody
    • DARPP-32 antibody
    • Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody
    • Dopamine and cAMP regulated neuronal phosphoprotein antibody
    • Dopamine and cAMP regulated phosphoprotein antibody
    • Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody
    • Dopamine and cAMP regulated phosphoprotein DARPP32 antibody
    • Dopamine- and cAMP-regulated neuronal phosphoprotein antibody
    • FLJ20940 antibody
    • IPPD antibody
    • Neuronal phosphoprotein DARPP 32 antibody
    • PPP1R1B antibody
    • PPR1B_HUMAN antibody
    • Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody
    • Protein phosphatase 1 regulatory inhibitor subunit 1B antibody
    • Protein phosphatase 1 regulatory subunit 1B antibody
    see all

图片

  • All lanes : Anti-DARPP32 antibody (ab51139) at 1/500 dilution

    Lane 1 : extracts from 293 cells treated with EGF
    Lane 2 : extracts from 293 cells treated with EGF with immunizing peptide

    Lysates/proteins at 0.2 µg/ml per lane.


    Predicted band size : 32 kDa
    Observed band size : 32 kDa
  • Ab51139 at 1/50 dilution staining human brain tissue, with and without blocking peptide; paraffin embedded.

文献

ab51139 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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