存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
纯度 Immunogen affinity purified
纯化说明 ab63396 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000.
1/500 - 1/1000. Predicted molecular weight: 32 kDa.
功能 May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.
组织特异性 Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells.
序列相似性 Contains 1 PH domain. Contains 1 SH2 domain.
翻译后修饰 Phosphorylated on tyrosine residues.
细胞定位 Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
Information by UniProt
B cell adapter molecule of 32 kDa antibody
B lymphocyte adapter protein Bam32 antibody
B-cell adapter molecule of 32 kDa antibody
Anti-DAPP1 (phospho Y139) antibody 图像
Immunocytochemistry/ Immunofluorescence - DAPP1 (phospho Y139) antibody (ab63396)
ab63396, at 1/500 dilution, staining DAPP1 in A549 cells by Immunofluorescence, in the absence (left image) or presence (right image) of the immunising peptide.
Western blot - DAPP1 (phospho Y139) antibody (ab63396)
All lanes : Anti-DAPP1 (phospho Y139) antibody (ab63396) at 1/500 dilution Lane 1 : 293 cell extracts treated
with Insulin (0.01U/ml, 2mins) Lane 2 : 293 cell extracts treated
with Insulin (0.01U/ml, 2mins) with immunising peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 32 kDa Observed band size : 27 kDa ( why is the actual band size different from the predicted?) Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
Anti-DAPP1 (phospho Y139) antibody (ab63396)参考文献
has not yet been referenced specifically in any publications.
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