Anti-DAPP1 (phospho Y139)抗体(ab63396)


  • 产品名称Anti-DAPP1 (phospho Y139)抗体
    参阅全部 DAPP1 一抗
  • 描述
    兔多克隆抗体to DAPP1 (phospho Y139)
  • 特异性ab63396 detects endogenous levels of DAPP1 only when phosphorylated at tyrosine 139.
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human DAPP1 around the phosphorylation site of tyrosine 139 (S-I-YP-E-S).

  • 阳性对照
    • WB: 293 cell extracts treated with Insulin (0.01U/ml, 2mins). ICC/IF: A549 cells.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab63396 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab63396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Predicted molecular weight: 32 kDa.
ELISA 1/5000.


  • 功能May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.
  • 组织特异性Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells.
  • 序列相似性Contains 1 PH domain.
    Contains 1 SH2 domain.
  • 翻译后修饰Phosphorylated on tyrosine residues.
  • 细胞定位Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B cell adapter molecule of 32 kDa antibody
    • B lymphocyte adapter protein Bam32 antibody
    • B-cell adapter molecule of 32 kDa antibody
    • BAM32 antibody
    • DAPP1 antibody
    • DAPP1_HUMAN antibody
    • DKFZp667E0716 antibody
    • Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide antibody
    • Dual adaptor of phosphotyrosine and 3 phosphoinositides antibody
    • hDAPP1 antibody
    see all

Anti-DAPP1 (phospho Y139) antibody 图像

  • ab63396, at 1/500 dilution, staining DAPP1 in A549 cells by Immunofluorescence, in the absence (left image) or presence (right image) of the immunising peptide.
  • All lanes : Anti-DAPP1 (phospho Y139) antibody (ab63396) at 1/500 dilution

    Lane 1 : 293 cell extracts treated with Insulin (0.01U/ml, 2mins)
    Lane 2 : 293 cell extracts treated with Insulin (0.01U/ml, 2mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 32 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

Anti-DAPP1 (phospho Y139) antibody (ab63396)参考文献

ab63396 has not yet been referenced specifically in any publications.

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